甘宝胶囊对小鼠急性肝损伤的保护作用

Protective Effects of Ganbao Capsule on Liver Injury in Mice

摘要:

目的: 研究甘宝胶囊对四氯化碳(CCl₄)、乙醇、扑热息痛所致小鼠急性肝损伤的保护作用。 方法: 取昆明种小鼠60只,随机分为6组:空白对照组,肝损伤模型对照组,甘宝胶囊0.125,0.25,0.5 g·kg^-1剂量组,联苯双酯阳性对照组0.2 g·kg^-1;ig给药,每日1次,至设定日期末分别用CCl₄、乙醇、扑热息痛造成急性肝损伤模型。比色法检测血清中的丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)和碱性磷酸酶(AKP)的水平,同时测定肝脏系数,此外CCl₄造模组测溶血素、脾脏和胸腺系数。 结果: 甘宝胶囊高、中剂量组能明显降低由CCl₄、乙醇、扑热息痛致肝损伤模型的ALT,AST,肝脏系数(P<0.05或P<0.01)。高剂量组能显著降低扑热息痛造模小鼠AKP的活性(P<0.05),并显著升高CCl₄造模小鼠的溶血素含量,降低胸腺系数及脾脏系数(P<0.05或P<0.01)。 结论: 甘宝胶囊对四氯化碳、乙醇、扑热息痛致肝损伤具有明显的保护作用。

Abstract:

Objective:To study the protect effects of Ganbao capsule on liver-injury induced by carbon tetrachlotide(CCl₄)ethanol and acetaminophen in mice. Method: Sixty KM mice were divided into 6 groups randomly: blank control group, model group with liver injury, three experimental groups (Ganbao capsule 0.125, 0.25, 0.5 g·kg^-1 respectively), positive control group (biphenyl double ester 0.2 g·kg^-1). CCl₄, ethanol and acetaminophen were accordingly given to mice by oral gavage once in order to make the acute liver injury model at the end of experiment. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (AKP) were measured, the liver index was simultaneously determined. In addition in CCl₄-induced model, hemolysin, spleen and thymus indexes were measured. Result: Ganbao capsule high, middle dose could obviously reduce ALT, AST, liver index for the liver injury models(P<0.05 or P<0.01). High dose could reduce AKP's content in the mice model induced by acetaminophen(P<0.05), and significantly increase hemolysin content in the CCl₄ model, and reduce the thymus index and spleen index in the same model(P<0.05 or P<0.01). Conclusion: Ganbao capsule has the function of protecting the liver from liver injury induced by CCl₄, ethanol and acetaminophen.