复荣通脉胶囊对糖尿病大鼠血清SOD, MDA水平及坐骨神经NGF表达的影响

Effects of Furong Tongmai Capsule on Plasma SOD, MDA and the Expression of NGF in Sciatic Nerve in Diabetic Rats

摘要:

目的: 观察复荣通脉胶囊对糖尿病大鼠血清超氧化物歧化酶(SOD)、丙二醛(MDA)及坐骨神经神经生长因子(NGF)的影响,探讨其对糖尿病周围神经病变(DPN)可能的作用机制。 方法: 将Wistar大鼠随机分为对照组和糖尿病造模组,造模成功的大鼠随机分为模型组、复荣通脉0.7,1.4,2.2 g·kg^-1组,观察8周后血糖、SOD,MDA水平及坐骨神经NGF免疫组化表达等。 结果: 8周后,糖尿病各组大鼠与对照组比较血糖均显著升高(P<0.01),血清SOD水平显著降低(P<0.01,P<0.05),MDA水平显著升高(P<0.01);复荣通脉1.4,2.2 g·kg^-1组与模型组、复荣通脉0.7 g·kg^-1组比较,SOD水平显著升高(P<0.01),MDA水平显著降低(P<0.05)。对坐骨神经NGF免疫组化分析发现,模型组、复荣通脉0.7 g·kg^-1组与对照组比较,NGF阳性率显著降低(P<0.01),复荣通脉1.4,2.2 g·kg^-1组与模型组、复荣通脉0.7 g·kg^-1组比较,NGF阳性率显著增加(P<0.05)。 结论: 复荣通脉胶囊能拮抗氧化应激,提高坐骨神经NGF水平。

Abstract:

Objective:To observe the effects of Furong Tongmai capsule (FTC) on the plasma superoxide dismutase(SOD),malondialdehyde (MDA)levels and the expression of nerve growth factor(NGF) in sciatic nerve in diabetic rats, and to explore possible mechanism on diabetic neuropathy. Method: Wistar rats were randomly divided into normal group and diabetic groups. The diabetic rats were randomly divided into model group,FTC groups (0.7,1.4, 2.2 g·kg^-1 accordingly). After treated 8 weeks, levels of plasma glucose,SOD, MDA were observed. The NGF of sciatic nerve was detected by immuneoshistochemistry array. Result: The levels of blood glucoses in diabetic rats were significantly higher than that in control group. Compared with model group, FTC 1.4, 2.2 g·kg^-1 groups showed increase in SOD were significantly. Higher level of SOD was found in FTC 1.4, 2.2 g·kg^-1 groups than those in the model group, while the levels of MDA were lower in the FTC 1.4, 2.2 g·kg^-1 groups than in the model group. By analysing the NGF of sciatic nerve, the positive rate of NGF expression in sciatic nerve was significantly lower in model and FTC 0.7 g·kg^-1 group than that in the control group. The positive rate of NGF expression was significantly higher in the FTC 1.4, 2.2 g·kg^-1 groups than those in the model and FTC 0.7 g·kg^-1 group. Conclusion: FTC may antagonise oxidative stress by increasing plasma SOD level and reducing plasma MDA level. FTC could increase NGF expression in sciatic nerve in diabetic rats.