葛根芩连汤抗大鼠心肌缺血再灌注致心律失常作用及其机制研究

Effect and Mechanism of Gegen Qinlian Decoction on Anti-arrhythmia Induced by Myocardial Ischemia Reperfusion in Rats

摘要:

目的: 研究葛根芩连汤对大鼠心肌缺血再灌注所致心律失常的治疗作用及其机制。 方法: 健康SD大鼠随机分为6组,每组10只,即假手术组、模型组、稳心颗粒组(2.43 g·kg^-1)及葛根芩连汤高、中、低(生药含量)(8.64,4.32, 2.16 g·kg^-1)剂量组,连续给药7 d后,除正常组外,其余各鼠结扎冠状动脉左前降支(LAD)建立大鼠心肌缺血再灌注致心律失常模型,监测肢体II导联心电图(ECG),Na⁺-K⁺-ATP酶(Na⁺-K⁺-ATPase),Ca²⁺-ATP酶(Ca²⁺-ATPase)及血清超氧化物歧化酶(SOD)活性,丙二醛(MDA)的含量。 结果: 葛根芩连汤能显著降低再灌注心律失常的发生率(P<0.01或 P<0.05);也能显著增加心肌组织匀浆Na⁺-K⁺-ATPase, Ca²⁺-ATPase活力(P<0.01或 P<0.05),增加血清SOD活性(P<0.01或 P<0.05),降低MDA含量(P<0.01或 P<0.05)。 结论: 葛根芩连汤能够有效的调节心肌缺血再灌注模型大鼠各种酶活性,清除氧自由基,从而改善心律失常,保护缺血心肌。

Abstract:

Objective: To study the effect and mechanism of Gegen Qinlian decoction on myocardial ischemia reperfusion induced arrhythmia in rats, to provide experimental evidence for clinical application. Method: Healthy SD rats were randomly divided into six groups (n=10, each), sham operation group (given equal volume distilled water), model group (given equal volume distilled water), Wenxin keli group (2.43 g·kg^-1), Gegenqinlian decoction high, medium, low dose (8.64, 4.32, 2.16 g crude drug·kg^-1) groups. Beside the sham operation group, the other groups were performed by liquating anterior interventricular branch of the left desending coronary artery to establish myocardial ischemia reperfusion induced arrhythmia. After different intervention for continuous 7 days, electrocardiogram of lead II was examined, and tissue Na⁺-K⁺-ATP enzyme (Na⁺-K⁺-ATPase), Ca²⁺-ATP enzyme (Ca²⁺-ATPase) and serum superoxide dismutase (SOD) and malondialdehyde (MDA) were determined. Result: Gegenqinlian decoction could significantly reduce the incidence of arrhythmia, serum MDA and increase serum SOD, also significantly increase Na⁺-K⁺-ATPase, Ca²⁺-ATPase activity (P<0.01 or P<0.05). Conclusion: Gegenqinlian decoction has good anti-arrhythmia effect in the way of increasing enzymes activity and removing oxygen free radicals, to improve arrhythmia and protect the ischemic myocardium.