咽舒宁颗粒祛痰镇痛及抑菌作用

Expectorant,Analgesic and Antibacterial Effects of Yanshuning Granules

摘要:

目的: 观察咽舒宁颗粒的祛痰、镇痛、抑菌等药效。 方法: 将小鼠随机分为对照、阳性药和咽舒宁颗粒高、中、低剂量组(11.00,5.50,2.75 g·kg^-1),ig 7 d,采用小鼠气管酚红排泌实验观察其祛痰作用,小鼠热板实验和醋酸扭体实验观察其镇痛作用,采用体外抗菌实验观察其对相关细菌的抑菌作用。 结果: 咽舒宁颗粒可增加小鼠离体气管酚红排泌量(P<0.05或P<0.01);提高热板所致小鼠的痛阈值(P<0.05);对抗醋酸所致小鼠扭体次数增加(P<0.05或P<0.01);对金黄色葡萄球菌[最小抑菌浓度(MIC)·mL^-1,最小杀菌浓度(MBC) 0.500 g·mL^-1]、甲型溶血性链球菌(MIC 0.062 g·mL^-1,MBC 0.250 g·mL^-1)、乙型溶血性链球菌(MIC 0.031 g·mL^-1,MBC 0.062 g·mL^-1)、变形杆菌(MIC 0.125 g·mL^-1,MBC 0.250 g·mL^-1)、肺炎链球菌(MIC 0.125 g·mL^-1,MBC 0.500 g·mL^-1)有较强的体外抑菌作用。 结论: 咽舒宁颗粒具有明显的祛痰、镇痛和抑菌作用。

Abstract:

Objective:To study the expectorant,analgesic and antibacterial effects of Yanshuning granules. Method: Mice were divided into 6 groups randomly: normal control group, positive groups, high dose group, middle dose group and low dose group of Yanshuning granules (11.00,5.50,2.75 g·kg^-1), after 7 d by ig administration,the expectorant effect was studied by a mouse model of phenol red test. The analgesic effect was studied by mouse models of hot-plate induced pain and acetic acid-induced writhing reaction.Experimental research on antibacterial action in vitro of Yanshuning granules was applied to observe the antibacterial effect. Result: The experimental results showed that Yanshuning granules could significantly increase phenol red excretion in mice (P<0.05 or P<0.01). It could significantly prolong the latent time of reaction on hot-plate in mice(P<0.05), reduce the writhing times in mice(P<0.05 or P<0.01).The granules showed obvious antibacterial actions against Staphylococcus aureus[minimal inhibitory loncentration(MIC) is 0.250 g·mL^-1, minimal bactericidal concentration(MBC) is 0.500 g·mL^-1], A-hemolytic streptococcus(MIC is 0.062 g·mL^-1,MBC is 0.250 g·mL^-1),Beta hemolytic streptococcus(MIC is 0.031 g·mL^-1,MBC is 0.062 g·mL^-1),Proteus(MIC is 0.125 g·mL^-1,MBC is 0.250 g·mL^-1) and Streptococcus pneumoniae(MIC is 0.125 g·mL^-1,MBC is 0.500 g·mL^-1). Conclusion: The granules has obvious expectorant,analgesic and antibacterial effects.