四逆汤对实验性高脂血症合并动脉粥样硬化兔高、低密度脂蛋白及载脂蛋白Apo-A, B含量的影响

Effect of Sini Decoction on HDL-CH, LDL-CH and Apo-A, B on Hyperlipidemia Combined with Atherosclerosis in Rabbits

摘要:

目的: 通过系统观察四逆汤干预对实验兔高脂血症(HLP)合并动脉粥样硬化(AS)模型的高、低密度脂蛋白胆固醇(HDL-CH,LDL-CH)和载脂蛋白A,B(Apo-A,Apo-B)的影响,以期探寻四逆汤治疗HLP及干预AS形成的作用机制。 方法: 采用高脂饲料喂养及耳缘静脉注射牛血清蛋白、皮下注射卵清白蛋白法建立兔HLP合并AS模型并将其随机分组;分别应用四逆汤(高、中、低剂量)治疗方法,采用空白组、模型组及西药组(给予阿托伐他汀)作为对照;运用比色法测定干预后血中HDL-CH,LDL-CH含量的变化,免疫比色法测定干预后血中Apo-A,Apo-B含量的变化。 结果: ①LDL,Apo-B含量:四逆汤低、中剂量组指标值均小于模型组,但差异无统计学意义;四逆汤高剂量组指标值明显小于模型组,差异具有统计学意义(P<0.05)。②HDL,Apo-A含量,HDL/LDL比值,Apo-A/Apo-B比值:四逆汤各剂量组指标数值均高于模型组,但低、中剂量组与模型组差异无统计学意义,而高剂量组与模型组差异具有显著统计学意义(P<0.01)。 结论: 四逆汤能够降低血中LDL和Apo-B的含量并使HDL,Apo-A含量升高是其能调节实验性兔HLP并AS模型血脂代谢的作用机制。

Abstract:

Objective:To observe the effect of Sini decoction(it consist of Radix Aconiti Praeparata, Rhizoma Zingiberis and Radix Glycyrrhizae Preparata) on high density lipoprotein cholesteol(HDL-CH),low density hpoprotein cholested( LDL-CH) and apolipoprotein (Apo)-A, B on the hyperlipidemia (HLP) combined with atherosclerosis in rabbits. Method: The rabbits were fed with high fat food everyday and injected with BSA, egg-white protein to make HLP combined AS modle, then randomized them into model group and three dose groups treated by Sini decoction(high, medium and low) and Atorvastatin respectively. Colorimetric assay method was used to determine LDL-CH, HDL-CH levels and electrophoresis wa used to measure levels of apolipoprotein A and B changes. Result: Sini decoction high-dose group, decreased LDL and Apo-B level compared with the model group (P<0.05). Sini decoction hihg dose group incresed HDL,Apo-A content, HDL/LDL and Apo-A/Apo-B ratio compared with the model group (P<0.01). Conclusion: Sini decoction can increase HDL and Apo-A content, reduce the content of LDL and Apo-B, which may be one of the mechanisms of the anti-hyperlipidemia with atherosclerosis rationale by Sini decoction.