桂枝汤及其拆方对芍药苷溶出量的影响

Effects of Guizhi Decoction and Its Disassembled Prescriptions on Dissolution of Paeoniflorin

摘要:

目的: 研究桂枝汤拆方前后芍药苷的含量变化。 方法: 采用Diamonsil RP-C₁₈色谱柱(4.6 mm × 250 mm,5 μm),流动相乙腈-水,流速1.0 mL·min^-1,检测波长230 nm。 结果: 桂枝汤拆方前后芍药苷含量由高到低的顺序为去大枣组>去生姜组>全方组>去桂枝组>去甘草组,去甘草组、去桂枝组与全方组比较均有显著性差异;去生姜组和去大枣组与全方组比较无显著性差异(P>0.05)。 结论: 甘草、桂枝均有助于白芍中芍药苷的溶出,生姜和大枣对白芍中芍药苷的溶出影响不大。

Abstract:

Objective: To investigate effects of Guizhi decoction and its disassembled prescriptions on the content of paeoniflorin. Method: A gradient HPLC system was used in the quantitative analysis of paeoniflorin on Diamonsil RP-C₁₈ column (4.6 mm×250 mm,5 μm) with acetonitrile and water as mobile phase,the flow rate was 1 mL·min^-1and the detection wavelength was 230 nm. Result: The content of paeoniflorin had great changes comparing Guizhi decoction with its different disassembled prescriptions,the order of it was group 4>group 3>group 5>group 1>group 2;By comparing with group 5,group 2 and group 1 had significant differences(P<0.05),but group 3 and group 4 had not. Conclusion: Radix Ei Rhizoma Glycyrrhizae and Ramulus Cinnamomi were helpful to the dissolution of paeoniflorin from Radix Paeoniae Alba,but Rhizoma Zingiberis Recens and Fructus Jujubae had few effects on it.