正交试验法优选桑椹补脑膏的壳聚糖絮凝工艺

Optimization of Chitosan Flocculation Process for Sangshen Bunao Creams by Orthogonal Test

摘要:

目的: 优选桑椹补脑膏的絮凝除杂工艺。 方法: 以除杂率,2,3,5,4'-四羟基二苯乙烯-β-D-葡萄糖苷和总多糖保留率的综合评分为指标,选取药液比、温度、壳聚糖用量及pH为考察因素,采用L₉(3⁴)正交试验法优选桑椹补脑膏的絮凝除杂工艺。 结果: 最佳絮凝工艺为药液比1:2,温度50℃,每100 mL药液加 1%壳聚糖溶液10 mL,pH 4。 结论: 该絮凝工艺稳定可行,除杂率高,有效成分损失量少。

Abstract:

Objective: To optimize flocculation clarification technology of Sangshen Bunao creams. Method: With the rate of impurity removal, the retention rate of stilbene glucoside and polysaccharide as comprehensive evaluation indexes,taking the degree of concentration,pH value,temperature and chitosan dosage as factors,L₉(3⁴) orthogonal test was adopted to optimize flocculation clarification technology of Sangshen Bunao creams by comprehensively weighted grading method. Result: Optimum flocculation clarification process conditions were as followings:water extraction liquid concentrated to 1:2,flocculation temperature of 50℃,per 100 mL concentrated solution plus 1% chitosan solution 10 mL,pH of 4. Conclusion: This flocculation clarification technology was feasible and stable with high rate of impurity removal and high retention rate of active ingredients.