正交试验优化板蓝根总生物碱的提取工艺

Optimization of Extraction Process for Total Alkaloids in Isatis indigotica by Orthogonal Design

摘要:

目的: 优选板蓝根中总生物碱的超声提取工艺。 方法: 以总生物碱提取量为指标,采用L₉(3⁴)正交试验考察乙醇体积分数、超声时间,料液比对提取工艺的影响,确定最佳提取工艺。 结果: 各因素对超声提取工艺的影响顺序为乙醇体积分数>超声时间>料液比;最佳提取工艺为乙醇体积分数80%,提取时间20 min,料液比1:10。 结论: 优选的提取工艺稳定可行,提取率高。

Abstract:

Objective: To optimize ultrasonic extraction process of total alkaloids in Isatis indigotica. Method: With extraction amount of total alkaloids as index,effects of ethanol concentration,liquid-solid ratio,ultrasonic time on extraction technology was investigated by by L₉(3⁴) orthogonal test. Result: The influencing order of each factors on ultrasonic-assistant extraction process of total alkaloids was ethanol concentration>extraction time>liquid-solid ratio. Optimum extraction process was as following:extracted 20 min with 10 times the amount of 80% ethanol. Conclusion: This optimized technology was stable and feasible with high extraction rate.