玉竹中酸性多糖的分离纯化及单糖组成分析

Isolation, Purification and Monosaccharide Analysis of an Acidic Polysaccharide from Polygonatum odoratum

摘要:

目的: 对玉竹粗多糖进行分离纯化,并对所得均一多糖进行定性分析。 方法: 采用热水提取玉竹粗多糖,乙醇分级沉淀,收集乙醇体积分数为80% 条件下析出的多糖沉淀物。再经DEAE-52纤维素离子交换层析和Sephadex G-100分子筛色谱得到玉竹酸性多糖(POAP80)。应用紫外光谱、凝胶柱色谱、纸色谱分析POAP80的纯度,红外光谱和PMP柱前衍生化高效液相色谱分析其结构和单糖组成。 结果: 从玉竹粗多糖中分离纯化了1种酸性多糖POAP80,主要含有甘露糖、葡萄糖、半乳糖醛酸、半乳糖4种单糖,其摩尔比为0.93:2.65:29.38:3.47。 结论: POAP80为玉竹中新发现的均一组分多糖,上述方法提供了玉竹POAP80的一些结构信息,为进一步研究其结构和功能提供参考。

Abstract:

Objective: To isolate and purify the crude polysaccharide from Polygonatum odoratum and then to identify the chemical structure. Method: A polysaccharide-rich extract was obtained by hot water extraction from Polygonatum odoratum and purified by grading ethanol precipitation and collect the precipitate at 80% volume fraction of ethanol, were sequentially purified by DEAE-cellulose chromatography and Sephadex G-100 molecular sieve chromatography and an acidic polysaccharide fraction named POAP80 was obtained. The purity of POAP80 was identified by UV absorption spectrum, IR spectrum and paper chromatogram. The monosaccharide compositions of POAP80 were analyzed by infrared spectroscopy and PMP pre-column derivatization high performance liquid chromatography. Result: An acidic polysaccharide was isolated and purified from Polygonatum odoratum and it was mainly composed of mannose, glucose, galacturonic acid and galactose at a molar ratio of 0.93: 2.65: 29.38: 3.47. Conclusion: POAP80 was found in the Polygonatum odoratum for a novel component. The structure information POAP80 was obtained through the above-mentioned methods, which provides the basis for the further study of the constitution and function of POAP80.