龙葵药材质量标准

Quality Standard of Solanum nigrum

摘要:

目的: 修订和完善龙葵药材的质量标准。 方法: 依据2010年版《中国药典》附录药品标准研究方法对龙葵药材进行显微、薄层鉴别,对10批龙葵药材的水分、灰分、酸不溶性灰分进行检查,并测定其有效成分含量。 结果: 确定了龙葵药材的显微特征,并建立了其TLC鉴别方法;水分不得>10.0%,酸不溶性灰分不得>3.0%;澳洲茄碱进样量在1.002~20.04 μg内呈良好线性关系,澳洲茄边碱进样量在1.023~20.46 μg内呈良好线性关系。 结论: 不同产地所含澳洲茄碱与澳洲茄边碱的总量差异较大,建立的方法能准确反映龙葵的内在质量,可作为龙葵药材质量标准的修订内容。

Abstract:

Objective: To revise and improve quality standard of Solanum nigrum. Method: According to the research methods of pharmaceutical standard in 2010 version of 'Chinese Pharmacopoeia’ Appendix, S. nigrum was identified by TLC and microscopy.Moisture,ash and acid insoluble ash from 10 batches of S. nigrum were detected,and the content of active ingredients were determined. Result: Microscopic characteristics of S. nigrum was derermined,identification methods of TLC was established;The content of moisture could not more than 10%,acid insoluble ash could not more than 3.0%;Solasonine was linear in the range of 1.002-20.04 μg,but solamargine was linear in 1.023-20.46 μg. Conclusion: The total content of solasonine and solamargine from S. nigrum in different areas had significant difference.This established method could accurately reflect the inherent quality of S. Nigrum,which could be used as the amendments of quality standard for S. nigrum.