不同生产期苦玄参叶HPLC指纹图谱

HPLC Fingerprint of Leaf of Picria fel-tarrae in Different Growth Periods

摘要:

目的: 建立广西产不同生长期苦玄参叶的HPLC指纹图谱。 方法: 采用高效液相色谱法(HPLC),C₁₈柱(4.6 mm×250 mm,5 μm,Japan Shimadzu)分离分析,以乙腈-0.1%冰醋酸溶液为流动相,梯度洗脱,检测波长264 nm,柱温25℃,流速1 mL·min^-1,采用"中药色谱指纹图谱相似度评价系统"(2004A) 软件进行分析。 结果: 10批苦玄参叶的HPLC指纹图谱共标定17个共有峰,相似度均>0.9。 结论: 建立苦玄参叶质量控制方法,指导苦玄参药材的采收加工和规范化种植提供实验数据。

Abstract:

Objective: To study the HPLC fingerprint of leaf of Picria fel-tarrae in different growth periods. Method: High performance of liquid chromatography (HPLC) was adopted, the separation was performed on C₁₈ (4.6 mm×250 mm,5 μm) column with acetonitrile-0.1% aqueous acetic acid solution as mobile phase using a gradient elution; the detection wavelength was set at 264 nm, the column temperature was set at 25℃ and the flow rate was 1 mL·min^-1; the data calculation was performed with similarity evaluation system for chromatographic fingerprint of TCM (Version 2004A). Result: In the fingerprint,17 common peaks were confirmed and the similarity of 10 batches of leaf of Picria fel-tarrae. were above 0.9. Conclusion: The experiment result could provide experimental data for the quality control method of the leaf of Picria fel-tarrae. and for optimum collection time and actualizing standard planting of Picria fel-tarrae.