HPLC同时测定连翘药材及提取物中芦丁、连翘酯苷A和连翘苷的含量

Simultaneous Determination of Rutin, Forsythoside A and Phillyrin of Forsythiae Fructus and its Extract by HPLC

摘要:

目的: 建立连翘药材及提取物中芦丁、连翘酯苷A和连翘苷的含量测定方法。 方法: 应用高效液相色谱-二极管阵列检测法(HPLC-DAD),根据各类成分紫外吸收光谱的差异,分别在354,329,277 nm 波长下检测芦丁、连翘酯苷A和连翘苷。采用Sunfire C₁₈ (4.6 mm×150 mm, 5 μm)色谱柱,流动相为乙腈(A)-0.2%冰醋酸溶液(B),梯度洗脱(0 min,14%A;15 min,18%A;40 min,30%A),流速1 mL·min^-1,柱温40℃。 结果: 芦丁、连翘酯苷A和连翘苷分别在0.098~4.9,0.24~12.0, 0.1~5.0 μg线性关系良好,平均回收率分别为98.63%,98.97%,102.18%,RSD分别为7.94%,0.93%,1.63%。 结论: 方法精密度高、重复性好,能够应用于连翘药材及提取物的质量控制。

Abstract:

Objective: To establish a HPLC method for simultaneous determination of rutin, forsythoside A and phillyrin of Forsythiae Fructus and its extract. Method: The procedure of HPLC-PDA was performed on the chromatographic column of Sunfire C₁₈ (4.6 mm×150 mm, 5 μm), and the mobile phase in the gradient elution (0 min, 14%A; 15 min, 18%A; 40 min, 30%A) was acetonitrile(A)-0.2% acetic acid solution (B). The flow rate was 1.0 mL·min^-1; the temperature was kept at 40℃, and the detection wavelength was set at 354, 329 nm and 277 nm respectively. Result: Rutin, forsythoside A and phillyrin were linear in the range of 0.1-2.0, 0.12-2.4 μg and 0.05-1.0 μg respectively. The average recoveries were 98.63%, 98.97% and 102.18% respectively (RSD 7.94%, 0.93% and 1.63%). Conclusion: The method is accurate with higher repeatability, which can be used in the quality control of Forsythiae Fructus and its extract.