HPLC测定野木瓜中木犀草素的含量

Determination of Luteolin in Caulis et Folium Stauntoniae by HPLC

摘要:

目的: 建立野木瓜中木犀草素的含量测定方法。 方法: Dikma Diamonsil C₁₈色谱柱(4.6 mm×250 mm,5 μm),流动相乙睛-0.1%磷酸溶液(26:74),流速 1 mL·min^-1,检测波长350 nm,柱温室温。 结果: 线性范围0.067 75~2.168 μg(r=0.999 9),平均回收率为99.25%,RSD 1.65%,精密度RSD 2.22%,重复性RSD 1.24%,稳定性RSD 2.58%。 结论: 方法稳定、可靠,可作为该药材的质量控制方法。

Abstract:

Objective: To establish the method for determination of luteolin in Caulis et Folium Stauntoniae. Method: The chromatographic conditions was as follows: a Dikma Diamonsil C₁₈ (4.6 mm×250 mm,5 μm) column,a mobile phase of acetonitrile-0.1% H₃PO₄ (26: 74); and a UV detector at 350 nm. Result: Luteolin was linear in the ranger of 0.067 75-2.168 μg (r=0.999 9); the average recovery of 99.25% (RSD 1.65%), the precision with RSD 2.22%, repetition with RSD 1.24% and stability with RSD 2.58% were obtained. Conclusion: The method is accurate,reliable,and can be used for quality control of the production.