HPLC同时测定猴耳环消炎胶囊中没食子酸与槲皮素的含量

Determination of Gallic Acid and Quercetin in Pithecellobium Clypearia Antiphlogistic Capsule by HPLC

摘要:

目的: 建立高效液相色谱法同时测定猴耳环消炎胶囊中没食子酸与槲皮素的含量。 方法: 伊利特Hypersil BDS-C₁₈(4.6 mm×150 mm,5 μm)色谱柱进行色谱分离,以甲醇-磷酸溶液梯度洗脱,流速1 mL·min^-1,检测波长254 nm,柱温30℃,进样体积10 μL。 结果: 没食子酸和槲皮素分别在15.0~180(r=0.999 6),1.25~15.0 mg·L^-1(r=0.999 7)线性关系良好;平均回收率分别为98.2%和99.2%,RSD分别为0.7%和0.5%。 结论: 方法操作简便,精密度高,结果准确,可同时测定猴耳环胶囊中没食子酸与槲皮素的含量,可用于猴耳环胶囊的质量控制。

Abstract:

Objective: To establish a HPLC method for determination of gallic acid and quercetin in Houerhuan Xiaoyan Jiaonang. Method: The analytical column was Hypersil BDS-C₁₈(4.6 mm×150 mm, 5 μm), and the mobile phase was methanol-phosphate solution with the gradient elution. The flow rate was 1.0 mL·min^-1 and the detection wavelength was 254 nm. The column temperature was kept at 30℃, and the sample volumn was 10 μL. Result: A good linearity was obtained in the range of 15.0-180.0 mg·L^-1 (r=0.999 6) and 1.25-15.0 mg·L^-1 (r=0.999 7) for gallic acid and quercetin respectively, and the average recovery was 98.2% (RSD 0.7%) and 99.2% (RSD 0.5%) respectively. Conclusion: The method is convenient, accurate, precise and can be used in the content determination of gallic acid and quercetin in Pithecellobium clypearia antiphlogistic capsule at the same time, and can be used for the quality control of this capsule.