HPLC测定冯了性风湿跌打药酒中盐酸麻黄碱的含量

Determination of Ephedrine Hydrochloride in Fengliaoxing Fengshi Dieda Wine by HPLC

摘要:

目的: 建立HPLC测定冯了性风湿跌打药酒中盐酸麻黄碱含量测定方法。 方法: 色谱柱为大连依利特C₁₈(4.6 mm×200 mm, 5 μm),以乙腈-0.1%磷酸溶液(含0.1%三乙胺)(3:97)为流动相,流速1 mL·min^-1,检测波长205 nm,柱温30℃。 结果: 盐酸麻黄碱与样品中其他组分分离效果好,盐酸麻黄碱进样量在0.188~0.940 μg具有良好线性关系,平均回收率99.39%,RSD 1.38%(n=6)。 结论: 方法简便易行,准确可靠,所建的方法可用于冯了性风湿跌打药酒的质量控制。

Abstract:

Objective: To establish an HPLC method for determination of ephedrine hydrochloride in Fengliaoxing Fengshi Dieda wine. Method: The chromatographic column was Yilite C₁₈(4.6 mm×200 mm, 5 μm); the mobile phase was acetonitril-0.1% phosphoric acid (0.1% triethameamine) (3: 97); the flow rate was 1 mL·min^-1; the detecting wavelength was set at 205 nm, and the column temperature was kept at 30℃. Result: The linear range of ephedrine hydrochloride was 0.188-0.940 μg. The average recovery was 99.39% and RSD was 1.38% (n=6). Conclusion: The method established was accuracy, sensitive and simple. It can be used for quality control of Fengliaoxing Fengshi Dieda wine.