双益降糖方对2型糖尿病大鼠糖、脂代谢的影响

Effect of Shuangyi Jiangtang Preseription on Metabolism of Blood Glucose and Blood Lipids in Type 2 Diabetic Rats

摘要:

目的: 探讨双益降糖方对2型糖尿病大鼠血糖、血脂代谢的影响。 方法: 利用大鼠口服高脂饲料4周后结合ip STZ 40 mg·kg^-1 建立2型糖尿病大鼠模型,将其随机分为2型糖尿病模型组(T2DM)、罗格列酮阳性对照组(0.29 mg·kg^-1 ig)、双益降糖方高、低剂量组(4 000,1 000 mg·kg^-1,ig),并设正常对照组(NC)。实验治疗4周后,观察治疗期间及治疗后大鼠的一般情况,检测大鼠糖耐量、糖化血红蛋白(HbA1c)、血脂、空腹血清胰岛素(INS)。 结果: 双益降糖方高剂量治疗组大鼠多饮、多尿、精神状态均较模型组有一定程度的改善,餐后0.5,2 h血糖较T2DM组降低、血清胰岛素水平升高(P<0.05),虽然空腹血糖、糖化血红蛋白、血脂与模型组比较有一定的改善,但统计学未见显著差异。双益降糖方低剂量组、罗格列酮阳性对照组均可改善2型糖尿病大鼠一般状况,与模型组比较糖耐量、糖化血红蛋白、血脂明显降低(P<0.05),血清胰岛素升高(P<0.05),双益降糖方低剂量组、罗格列酮阳性对照组之间未见显著性差异。 结论: 双益降糖方可改善2型糖尿病大鼠糖、脂代谢及胰岛功能,其中以低剂量组作用最佳,其疗效与罗格列酮相似。

Abstract:

Objective: To investigate the effect of the Shuangyi Jiangtang prescription on the blood glucose and blood lipids in type 2 diabetic rats. Method: rats model of type 2 diabetes mellitus was established by taking high fat diet for 4weeks and combined with STZ ip for 40 mg·kg^-1,and the model rats were randomly divided into diabetes model group (T2DM), rosiglitazone maleate positivecontrol groups (RM,ig 0.29 mg·kg^-1·d^-1), Shuangyi Jiangtang prescription high dose group (SG,ig 4 000 mg·kg^-1·d^-1), Shuangyi Jiangtang prescription low dose group (SD ig 1 000 mg·kg^-1·d^-1), and the normal control group (NC). We had observed the general condition of rats during and after the whole treatment of 4 weeks, and tested glucose tolerance, glycated hemoglobin(HbA1c), blood lipids, and fasting serum insulin (INS). Result: In the group of SG, the rats’ symptoms of polydipsia, polyuria and mental status were improved in some degree,fasting blood glucose(FBG), 0.5 h blood glucose(BG) and 2 h BG were lower than the T2DM group,the fasting serum insulin level was higher than the T2DM group (P<0.05). Compared with the model group, fasting blood glucose, glycosylated hemoglobin, lipids were improved in some degree, but there was no significant statistics difference. In the groups of SD and RM, the general condition of rats were bettered, and compared with the model group, the glucose tolerance, HbA1c and the blood lipid of the rats decreased significantly(P<0.05), and the serum insulin increased significantly(P<0.05), but between the two groups of SD and RM, there was no difference in those indexes. Conclusion: The Shuangyi Jiangtang prescrription can improve sugar, lipid metabolism and islet cells function of type 2 diabetic rats,the low-dose group has the best effect, and its efficacy is similar to the rosiglitazone maleate.