姜炭对虚寒性出血证大鼠凝血功能, 血栓素B2和 6-酮-前列腺素F1α的影响

Investigation of Zingiber officinale Carbo on Coagulation functionand TXB2, 6-keto-PGFla in Asthenia Cold and Blood Deficiency Syndrome Rats

摘要:

目的: 观察姜炭对虚寒性出血证大鼠凝血5项指标和血栓素B₂(TXB₂)、6-酮-前列腺素F_1α(6-keto-PGF1α)的影响。 方法: 将80只SD大鼠随机分为8组:空白组、模型组、姜炭组和姜炭加模型组高、中、低剂量组,每组10只。模型组和姜炭加模型组均以大黄水煎液ig造成虚寒模型,共10 d。从造模第6天开始,空白组和模型组ig生理盐水,姜炭组ig给与不同剂量的姜炭混悬液(10,20,40 g·kg^-1),1 次/d,连续5 d;模型组和姜炭组采用眼眶放血法造成出血模型。末次给药后1 h采血测定凝血5项:凝血酶时间(TT)、凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、纤维蛋白原(FIB)、D-二聚体(D-D)和血栓素B₂(TXB₂)和6-酮-前列腺素F1α(6-keto-PGF_1α)。 结果: 姜炭高、中剂量组能显著缩短虚寒性出血证大鼠的TT(P<0.05),PT(P<0.05),APTT(P<0.05),提高FIB的含量(P<0.01)、减少D-D的含量 (P<0.05),提高TXB₂(P<0.01)而下调6-keto-PGF1α(P<0.01)的含量。 结论: 姜炭可能是通过影响大鼠凝血系统和调节TxB₂,6-酮-前列腺素F_1α含量来发挥止血、凝血作用。

Abstract:

Objective: To investigate the effects of Zingiber officinale carbo on coagulation function and thromboxane B₂(TXB₂), 6-keta-prostaglandin F_1α(6-keto-PGF_1α) in asthenia cold and blood deficiency syndrome Rats. Method: Eighty SD rats were randomly divided into 8 groups: normal control,model,small, medium and large dose groups of Z. officinale carbo.The model of asthenia cold was made by oral administration of rhubarb for 10 days.After 6 days of model, the normal control group and the model group were ig given normal saline.Z. officinale carbo groups were given the Z. officinale carbo decoction at different doses (10,20,40 g·kg^-1) daily for 5 days.Blood deficiency syndrome was made by the orbit blooding method. 1 h after lasting administration, blood samples were collected to detect the serum levels of thrombin time(TT), prothrombin time(PT), activated thromboplastin time(APTT), fibrinogen(FIB), d-dimer(D-D) and TXB₂/6-keto-PGF_1α were observed. Result: Z. officinale carbo could effectively shorten TT(P<0.05), PT(P<0.05), APTT(P<0.05), increase the contents of FIB(P<0.01), and decrese the contents of D-D(P<0.05), increase the level of TXB₂ (P<0.01) and decrease the level of 6-keto-PGF_1α(P<0.01). Conclusion: The mechanism of Z. officinale carbo may affect the coagulation and the level of TXB₂ and 6-keto-PGF_1α.