升清降浊方对谷氨酸钠肥胖模型大鼠骨骼肌糖脂代谢的影响

Effect of Shengqing Jiangzhuo Fang on Glycolipid Metabolism in Monosodium L-Glutamate Rats

摘要:

目的: 观察升清降浊方对皮下注射谷氨酸钠(MSG)大鼠骨骼肌的影响,以探索其改善胰岛素抵抗的作用机制。 方法: 新生大鼠皮下注射MSG并高脂诱导,制造胰岛素抵抗肥胖大鼠,选择48只MSG肥胖模型大鼠,雌雄各半。将大鼠随机分为6组:模型组,菲诺贝特100 mg·kg^-1剂量组,罗格列酮5 mg·kg^-1剂量组,升清降浊方6,3,1.5 g·kg^-1剂量组;选正常鼠8只,雌雄各半做为正常对照组。给药50 d后,观察受试药物对MSG肥胖模型大鼠骨骼肌中总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)及糖元含量的影响。 结果: 升清降浊方对MSG肥胖模型大鼠骨骼肌糖脂代谢有明显改善作用,与模型组相比,给药组大鼠骨骼肌中TC,TG,LDL-C及糖元含量明显降低,升清降浊方3个剂量组具有极显著性差异(P<0.01);给药组骨骼肌中HDL-C含量明显升高,升清降浊方3 g·kg^-1剂量组具有极显著性差异(P<0.01)。 结论: 升清降浊方能明显改善MSG大鼠骨骼肌组织中脂质和糖原含量。

Abstract:

Objective: To observe the effects of Shengqing Jiangzhuo Fang(SQJZF) on skeletal muscle in monosodium L-glutamate(MSG) obese rats with insulin resistance. Method: The rat MSG-obese model was produced by giving neonatal rats subcutaneous injection of monosodium glutamate and induced with high-fat diet. Sixty MSG rats were divided into seven grous(4 male and 4 female per gtoup) randomly.the rosiglitazone group,the fenofibrate group,high dose,middle dose and low dose of SQJZF.After the administration for 50 days, levels of cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterin(LDL-C),high density lipoprotein cholesterin(HDL-C) and glucogn in muscle tissue were measured. Result: SQJZF showed good effects on the animal models. Compared with the model group,treatment with SQJZF at a dose of 6,3,1.5 g·kg^-1 for 50 d decreased levels of TC,TG,LDL-C and glucogn in muscle tissue (P<0.01);levels of HDL-C was significantly elvated by treatment with SQJZF at a dose of 3 g·kg^-1(P<0.01). Conclusion: SQJZF may contribute to ameliorate the glucolipid metablization in diabetic skeletal muscle.