大豆苷元对异丙肾上腺素所致心肌肥厚与抗氧化的影响

Anti-oxidation Effects of Daidzein on Myocardial Hypertrophy Caused by Isopropylarterenol

摘要:

目的: 建立异丙肾上腺素(isopropylarterenol,Iso)诱导的大鼠心肌肥厚模型,观察大豆苷元(daidzin,DD)对大鼠心肌肥厚时抗氧化功能的影响。 方法: 健康雄性SD大鼠随机分为5组:对照组、心肌肥厚模型组、溶剂对照组、大豆苷元低、高剂量(0.1,0.3 μmol·kg^-1)组。各组分别背部sc同体积NS或Iso 1.0 mg·kg^-1,每日1次,连续10 d,建立心肌肥厚模型。给Iso第2天开始ip给药,连续14 d。通过测定全心质量参数、左心室质量指数、血清丙二醛(MDA)含量、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性来判断大豆苷元对Iso所致心肌肥厚的保护作用。 结果: 与模型组相比,0.1 μmol·kg^-1大豆苷元组的全心质量参数(2.8±0.07)mg·g^-1及左心室质量指数(2.21±0.02)mg·g^-1明显降低(P<0.05,P<0.01)。与模型组相比,0.1, 0.3 μmol·kg^-1大豆苷元组MDA含量显著降低(12.2±1.6),(12.1±1.8) nmol·L^-1(P<0.01)。与模型组相比,0.3 μmol·kg^-1大豆苷元组的SOD活性显著升高(197.8±7.3)U·mL^-1(P<0.05)。与模型组相比,0.1,0.3 μmol·kg^-1大豆苷元组的血清GSH-Px活性均显著升高(496.7±30.4),(521.6±18.8)U·mL^-1,P<0.01)。 结论: 大豆苷元对异丙肾上腺素诱导的大鼠心肌肥厚具有保护作用,提高大鼠的抗氧化功能可能是大豆苷元抗心肌肥厚的机制之一。

Abstract:

Objective: To explore the anti-oxidation effects of daidzein (DD)on rat's myocardial hypertrophy caused by isopropylarterenol (Iso). Method: The rat's myocardial hypertrophy model was formed by hypodermic injection of Iso 1 mg·kg^-1)for days at back skin,DD (0.1, 0.3 μmol·kg^-1),dimethylsulfoxide(DMSO) or normal saline(NS) were administrated in intragastric way from the second day for 14 days. After the last administration, 12 hours after fasting blood and heart were collected The indexes of whole heart and left ventricle were calculated,superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px)activity and malondialdehyde (MDA) concentration in serum were measured. Result: It showed that the indexes of whole heart and left ventriclein Iso group were much bigger than control one,the activity of SOD and GSH-Px in serum was decreased, and the concentration of MDA in serum enhanced. However, DD improved these indexes. Conclusion: DD has a protection on rat's myocardial hypertrophy caused by Iso through enhancing the anti-oxidation activity of rats.