不同饲料配方对大鼠TC, LDL-C, Hcy的影响

Effect of Different Feeds on TC, LDL-C, Hcy in Rats

摘要:

目的: 探讨合适的饲料配方,在较短的实验周期内形成稳定、适用的高脂高同型半胱氨酸血症(hyperhomocysteinemia, HHcy)动物模型。 方法: 40只雄性SD大鼠分为4组,每组10只,分别按组给予普通饲料、配方1(基础饲料占88.5%,高脂高胆固醇不添加丙硫氧嘧啶)、配方2(较配方1增加5%蛋黄糖,5%全脂奶粉,0.15%丙硫氧嘧啶)、配方3(0.25%丙硫氧嘧啶,较配方2增加5%蛋黄粉)饲料常规喂养。每周称1次体重,观察体重变化。分别于2,4周取血测定血清中总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)变化及血浆同型半胱氨酸(homocysteine,Hcy)的浓度。 结果: 与对照组相比配方1组体重没有明显差异;配方2组,3组体重显著下降(P<0.01)。2,4周时与对照组相比,血清TC和LDL-C含量及血浆Hcy浓度,配方1组没有显著性差异;配方2组,3组都有显著升高(P<0.01)。 结论: 该实验研究调整后的大鼠饲料配方2组是比较合适的饲料配方组成,能在较短的时间内形成稳定的大鼠高脂HHcy血症动物模型。

Abstract:

Objective: We explore a practical feed formulation in order to establish a stable atherosclerosis animal model.With high fat and hyperhomocysteinemia in shorter period. Method: Forty male SD rats were divided into 4 groups (n=10), given different formula feeding with common feed,formulationⅠ(not containing propylthiouracil),formulation II(containing propylthiouracil of 0.15%) and formulation Ⅲ(containing propylthiouracil of 0.25%). weighed once a week.The blood samples were collected at week 2 and 4 respectively, then to determine the levels of total cholesterol(TC),low density lipoprotein cholesterin(LDL-C) in serum and homocysteine(Hcy) in plasma. Result: Compared with the control group, weight of formulationⅠhad no significant difference, while weight of formulation II and formulation Ⅲ decreased significantly (P<0.01);TC,LDL-C and Hcy levels in formulation II and formulation Ⅲ groups increased significantly (P<0.01) at 2, 4 weeks,respectively,but formulationⅠhave no significant difference. Conclusion: In this study,we found that the rats fed with the formulation II can establish a stable atherosclerosis animal model with high fat and hyperhomocysteinemia in shorter period.