不同提取工艺生殖营养方对腺嘌呤所致小鼠肾功能损伤的影响

Effects of Different Technologic Extracts from Shengzhi Yingyang Fang on Renal Function Poisoned by Adenine in Mice

摘要:

目的: 探讨不同提取工艺的生殖营养方对小鼠肾功能的影响。 方法: 100只雄性健康小鼠随机分为对照组、模型组、生殖营养方1(水提)、2(70%乙醇提)、3(40%乙醇提)共5组,模型与生殖营养方3组按300 mg·kg^-1腺嘌呤灌胃造模,每周2次;造模同时生殖营养方组分别灌胃不同提取工艺的生殖营养方煎剂,按生药量折算为1.96 g·kg^-1,每周5次,试验周期为2个月。 结果: 生殖营养方各提取物组显著降低腺嘌呤染毒小鼠血浆中的肌酐(SCr)、尿素氮(BUN)、乳酸(LD)含量及黄嘌呤氧化酶(XOD)的活性(P<0.05),显著降低肾组织中的酸性磷酸酶(ACP)、碱性磷酸酶(AKP)活性(P<0.05),并显著升高琥珀酸脱氢酶(SDH)活性及蛋白(Pr)浓度(P<0.05)。均以水提物的保护作用最好。 结论: 不同提取工艺的生殖营养方对腺嘌呤染毒造成的小鼠肾功能的损伤有保护作用,以水提效果最好。

Abstract:

Objective: To study effect of different technologic extracts from shengzhi Yingyang fang on renal function poisoned by adenine in mice. Method: One hundred SPF grade male healthy mice were randomly divided into 5 groups, which were the control group, the model group, Shengzhi Yingyang Fang 1 group(water extraction), Shengzhi Yingyang Fang 2 group(75% alcohol), Shengzhi Yingyang Fang 3 group(40% alcohol). The models were established by orally giving adenine to the mice at 300 mg·kg^-1 and at the same time, 3 experimental groups were orally given different technologic extracts from sheng zhiyingyang fang decoctum (crude drugs at 1.96 g·kg^-1), five times a week for 2 months. Result: Shengzhi Yingyang fang significantly decreased (P<0.05)the contents of creatinine (SCr),urea nitrogen(BUN),lactic acid(LD) and the activity of xanthine oxidase(XOD)in plasma. Furthermore, it could significantly reduce(P<0.05) the activities of acid phosphatase (ACP) and alkaline phosphatase (AKP) and improve(P<0.05)the activities of succinodehydrogenase (SDH) of renal tissue poisoned by adenine in mice. The effect of water extraction is the best. Conclusion: Different technologic extracts from shengzhi Yingyang fang decoctum have protective effects on renal function poisoned by adenine, among which the effect of water extract is the best.