新疆鼠李提取物对大鼠酒精性肝病的作用

Protective Effect of Rhamnus Songorica Extract on Rats with Alcoholic Liver Disease

摘要:

目的: 探讨新疆鼠李提取物对酒精性肝病大鼠的作用。 方法: 采用酒精灌胃法建立大鼠酒精性肝病动物模型,每天1次,连续10周。造模成功后,阳性对照组按照100 mg·kg^-1剂量给予益肝灵,新疆鼠李提取物低、中、高剂量组分别按照0.9, 1.8, 3.6 g·kg^-1口服给药。给药4周后,分别测定各组血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(LDL-C)和肝组织丙二醛(MDA)、总超氧化物歧化酶(T-SOD)、一氧化氮(NO)、还原型谷胱甘肽(GSH),并观察肝脏组织病理学改变。 结果: 与模型组比较,新疆鼠李提取物组血清中ALT, AST活性和TG, TC, LDL-C含量较模型组显著降低(P<0.01)。肝组织中MDA, NO含量较模型组显著降低(P<0.05),大剂量组T-SOD活性较模型组显著升高(P<0.05),肝组织病理改变明显好转。 结论: 新疆鼠李提取物通过增强机体清除自由基、抗脂质过氧化作用的能力改善脂质过氧化反应对肝脏的损伤和保护肝细胞的结构和功能,发挥对酒精性肝病的治疗作用。

Abstract:

Objective: To observe the therapeutic effects of Rhamnus Songorica extract (RSE) in rats with alcoholic liver disease. Method: A rat model of alcoholic liver disease was established by intragastric infusion of alcohol, once a day continuously for 10 weeks. Positive control group was given yiganling at 100 mg·kg^-1 and the low, middle, high dose RSE groups were given RSE at 0.9, 1.8, 3.6 g·kg^-1, respectively after 10 weeks. The levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C) in serum, maleic dialdehyde (MDA), total superoxide dismutase (T-SOD), nitric oxide (NO), reduced glutathione (GSH) in liver tissue and the evaluation of the histopathological changes of liver in rats were detected after 4 weeks treatment. Result: Compared with the rats in model group, RSE significantly decreased the levels of ALT, AST, TG, TC, and LDL-C in serum as well as MDA and NO in liver tissue. The T-SOD conten was obviously increased in liver tissue. The hepatic pathological changes were milder in the RSE groups than in the model group. Conclusion: RSE has obviously therapeutic effects on alcoholic liver disease in rat. The mechanisms may be relative to reducing lipid peroxide and protecting the structure of hepatocyte via enhancing the abilities of the free radical scavenging and antilipoperoxidant activities.