蒺藜两地汤对糖尿病模型大鼠的降血糖作用及外周神经保护作用

Protective and Alleviative Effects of Jili Liangdi Tang on Streptozotocin-induced Diabetes Mellitus and Peripheral Neuropathy in Rats

摘要:

目的: 研究蒺藜两地汤对链脲菌素(STZ)诱导的糖尿病模型大鼠的降血糖作用以及对糖尿病周围神经痛的保护作用。 方法: 大鼠60只随机分为正常对照组,模型组,阳性药二甲双胍组(200 mg·kg^-1)以及蒺藜两地汤低、中、高(含生药16.2,32.4, 64.8 g·kg^-1)剂量组,除正常组外各组大鼠ip STZ造2型糖尿病模型,造模成功后ig给予相应药物,连续4周。记录大鼠饮食饮水及体重的变化,检测大鼠血糖和胰岛素水平的变化。大鼠60只,随机分为正常对照组,模型组,阳性药加巴喷丁组(120 mg·kg^-1)以及蒺藜两地汤低、中、高(含生药16.2,32.4, 64.8 g·kg^-1)剂量组,除正常组外各组大鼠尾iv STZ 7 d造大鼠糖尿病外周神经痛模型,并ig相应药物,检测大鼠热辐射痛阈以及机械痛阈的变化。 结果: 蒺藜两地汤可以剂量依赖地减少糖尿病模型大鼠的饮食、饮水量,增加其体重,高剂量组使大鼠血糖水平降至10.3 mmol·L^-1,大鼠胰岛素的分泌增加0.61 μg·L^-1, 与模型组相比均有显著差异(P<0.05)。蒺藜两地汤各剂量组均可以升高大鼠的热辐射痛阈及机械痛阈,高剂量组对热痛及机械痛的镇痛率分别为50.3%,63.2%(P<0.05),表现出良好的镇痛效应。 结论: 蒺藜两地汤对糖尿病模型大鼠的相关症状具有一定的改善作用,可以升高大鼠体内胰岛素水平,并对糖尿病引起的周围神经痛具有保护作用。

Abstract:

Objective: To investigate the benefitial and protective effects of Jili Liangdi Tang (JLLDT) on Streptozotocin (STZ)-induced diabetes mellitus and periphery neuropathic pain. Method: Sixty rats were employed and divided into control, model, positive control (metformin 200 mg·kg^-1) and JLLDT low, medium and high dose (16.2, 32.4, 64.8 g·kg^-1) groups evenly. Except of the control group, all the rats were established type II diabetes mellitus induced by intraperitoneal injection of STZ. The rats were intragastric administration of corresponding agent for 4 weeks, while the intake of food and water were recorded. The blood glucose and insulin levels were also measured by blood glucose meter or ELISA kit. Sixty rats were employed and divided into control, model, positive control (gabapentin 120 mg·kg^-1) and JLLDT low, medium and high dose (16.2, 32.4, 64.8 g·kg^-1) groups evenly. Except of control group, all the rats were established diabetes mellitus neuropathy induced by intravenous injection of STZ for 7 days. The rats were intragastric administration of corresponding drugs, while the thermal hyperalgesia and mechanical allodynia were measured respectively. Result: JLLDT could decrease the food and water intake and elevate the body weight of diabetic rats significantly in a dose-dependent manner. The blood glucose concentration was deceased to 10.3 mmol·L^-1and insulin level was elevated to 0.61 μg·L^-1significantly after administration of JLLDT high dose. The three doses of JLLDT could ameliorate the thermal hyperagesia and allodynia of model rats respectively. The analygesia rate in JLLDT high dose reached at 50.3% and 63.2%, respectively (P<0.05). Conclusion: JLLDT exhibits pronounced ability of decrease diabetic blood glucose by elevating insulin secretion, and has protective effect in diabetic periphery neuropathic pain.