三七方的拆方研究

Prescription Research on Sanqi Fang

摘要:

目的: 探讨三七方组方的合理性,分析方中各药味对全方镇静催眠作用的影响。 方法: 本研究在中医理论指导下采用撤药拆方法进行拆方研究,将小鼠随机分为空白对照组、全方组(0.767 g·kg^-1)、去酸枣仁组(0.517 g·kg^-1)、去鸡血藤组(0.433 g·kg^-1)、去三七组(0.683 g·kg^-1)和去小蓟组(0.667 g·kg^-1)。各组连续灌胃给药11 d,空白对照组予同容积的蒸馏水,第11天进行行为学测试,自主活动和与戊巴比妥钠的协同作用。 结果: 与空白对照组比较,全方组和去鸡血藤组均能显著降低小鼠自主活动次数(P<0.05);全方组能显著增加小鼠的入睡率(P<0.05);去鸡血藤组和去小蓟组有增加小鼠入睡率的趋势;全方组(P<0.05)和去三七组(P<0.01)均能明显缩短小鼠的入睡时间,去小蓟组有缩短小鼠入睡时间的趋势;全方组、去小蓟组和去三七组均能显著增加小鼠的睡眠时间(P<0.05)。 结论: 三七方具有确切的镇静催眠作用,组方合理。酸枣仁(制)、鸡血藤、三七和小蓟均影响着全方镇静催眠作用的发挥,是三七方不可或缺的组成部分。

Abstract:

Objective: To analyze the rationality of Sangi fang. Method: Rats were divided randomly into control group, Sanqi fang group(0.767 g·kg^-1), Sanqi fang without Ziziphi Spinesae Semen group(0.517 g·kg^-1), Sanqi fang without Spatholobi Caulis group(0.433 g·kg^-1), Sanqi fang without Notoginseng Radix et Rhizoma group(0.683 g·kg^-1) and Sanqi fang without Cirsii Herba group (0.667 g·kg^-1). All of rats in therapeutic groups were administered the medication onally for 11 days and the rats in blank control group were given the distilled water with same volume. On the 11th day, the behavior test was performed including spontaneous movement and cooperating function with Pelltobarbitalum Natricum. Result: Compared to the control group, Sanqi fang and Sanqigang without Spatholobi Caulis group could remarkably reduce the degree of rat's spontaneous movement(P<0.05); Sanqi fang group could increase the sleeping rate significantly(P<0.05). While both Sanqi fang without Spatholobi Caulis and without Cirsii Herba group could increase the tendency of sleeping rate. Sanqi fang goup(P<0.05) and Sanqi fang without Sanqi group(P<0.01) could reduce fall-sleep time significantly, while Sanqi fang without Cirsii Herba group had the tendency of reduce fall-sleep time. Sanqi fang group, Sanqi fang without Cirsii Herba group and Sanqi fang without Notoginseng Radix et Rhizoma group can increase sleeping time of rats significantly(P<0.05). Conclusion: Sanqi fang has sedative-hypnotics effect, and its the prescription formula is reasonable.