雷公藤内酯醇对神经胶质瘤侵袭的体外抑制作用

Anti-invation Effect of Triptolide for U87 Glioma in vitro

摘要:

目的: 研究雷公藤内酯醇对胶质瘤侵袭性的体外抑制作用,并探讨其作用分子机制。 方法: 通过MTT法测定雷公藤内酯醇对胶质瘤细胞株U87细胞增殖抑制作用,划痕实验和Transwell实验评价雷公藤内酯醇对细胞侵袭能力的影响,Real-Time PCR和Western印迹法检测雷公藤内酯醇对U87胶质瘤细胞的组织蛋白酶B(cathepsins B, CB)、基质金属蛋白酶9(MMP-9)和核因子NF-κB (p65)表达的影响。 结果: 雷公藤内酯醇对胶质瘤细胞增殖有显著的抑制作用,同时可降低U87胶质瘤细胞的迁移和侵袭能力,下调其CB,MMP-9和NF-κB表达水平。 结论: 雷公藤内酯醇降低U87胶质瘤细胞侵袭能力的机制可能与抑制NF-κB通路、下调CB及MMP-9的表达、降低恶性胶质瘤水解细胞外基质的能力有关。

Abstract:

Objective: To study the anti-invation effct of triptolide for U87 glioma cells and its molecular mechanism. Method: MTT assay was used to determine the growth inhibition by triptolide in glioma U87 cells. Tumor invasion and migration were examined by Transwell chamber assay and wound-healing method. Real-time PCR was conducted to detect alteration of matrix metallopeptidase 9 (MMP-9) and Cathepsins B (CB). The alteration of nuclear factor kappa B (NF-κB) p65 expression were studied by Western blot. Result: Triptolide could significantly inhibite glioma U87 cell proliferation, reduce the cell ability of migration and invasion, down-regulating its MMP-9, CB and NF-κB p65 expression level. Conclusion: Triptolide could depress the invasion of glioma cells owing to down-regulating the level of NF-κB pathway which could inhibit MMP-9 activities, reduce the ability of hydrolyzing extracellular matrix in vitro.