黄连解毒汤对耐药性大肠杆菌的逆转作用

Reversal Effect of Huanglian Jiedu Tang for Drug Resistance in Escherichia coli

摘要:

目的: 探讨黄连解毒汤对耐药性大肠杆菌逆转作用的影响。 方法: 采用纸片法筛选产超广谱β-内酰胺酶大肠杆菌,确定黄连解毒汤的最低抑菌质量浓度,选取低于最低抑菌浓度的黄连解毒汤培养超广谱β-内酰胺酶大肠杆菌,确定最佳逆转效果的培养时间和药物浓度,并测定逆转率。 结果: 黄连解毒汤对大肠杆菌ATCC8099最低抑菌浓度为0.6 g·L^-1,逆转超广谱β-内酰胺酶大肠杆菌的最佳培养时间为30 h,最佳质量浓度为0.3 g·L^-1,对氨苄西林药敏逆转率为60.0%、头孢他啶药敏逆转率为66.7%、头孢美唑药敏逆转率为75.0%,头孢曲松药敏逆转率为66.7%。 结论: 黄连解毒汤对耐药性大肠杆菌逆转作用确切,可作为逆转耐药性大肠杆菌一种途径。

Abstract:

Objective: To study the effect of Huanglian Jiedu Tang (HJT) in the reversal effect of drug resistance in Escherichia coli. Method: E. coli with extended-spectrum β-lactamase is screened by disc diffusion method. The minimum inhibitory concentration of HJT was determined.The extended-spectrum β-lactamase enzyme E. coli was cultured with a HJT concentration lower than the minimum inhibitory concentration. The cultivation duration and optimal concentration for reversal effect were selected, and the reversal rate was determined. Result: Minimum inhibitory concentration of HJT to the E. coli ATCC8099 was 0.6 g·L^-1. The cultivation duration was 30 h and the optimal concentration for reversal effect was 0.3 g·L^-1.The reversal rate of ampicillin was 60.0%. The reversal rate of ceftazidime was 66.7%. The reversal rate of cefmetazole was 75.0%. The reversal rate of ceftriaxone was 66.7%. Conclusion: The effect of HJT for reversal drug resistance in E. coli is defined. It is a way to reverse drug resistance in E. coli.