龙利叶抗炎镇痛作用研究

Anti-inflammatory and Analgesic Effect of Sauropus rostratus

摘要:

目的: 研究龙利叶水提液的抗炎和镇痛作用。 方法: 采用改良寇氏法对其急性毒性进行研究;采用二甲苯致小鼠耳廓肿胀、角叉菜胶致大鼠足跖肿胀、小鼠棉球肉芽肿增生模型,评价龙利叶水提液(含生药14,7,3.5 g·kg^-1)的抗炎作用,采用扭体法和热板法评价其镇痛作用。 结果: 龙利叶水提液的LD₅₀为68 g·kg^-1,对二甲苯致小鼠耳廓肿胀、棉球致小鼠肉芽肿和角叉菜胶致大鼠足跖肿胀均有明显的抑制作用(P<0.05或P<0.01),能显著减少醋酸致小鼠扭体反应的次数(P<0.05或P<0.01),但不能提高小鼠的痛阈。 结论: 龙利叶水提液具有抗炎和镇痛作用, 为龙利叶的开发和利用提供了科学依据。

Abstract:

Objective: To study anti-inflammatory and analgesic effect of water extract of Sauropus rostratus. Method: The LD₅₀level of the water extract was determined by modified Karber's method. Dimethylbenzene-induced auricle edema in mice,carrageenan-induced hind paw edema in rats and cotton ball-induced granuloma in mice were respectively performed to investigate the anti-inflammatory effect of the extract (14,7,3.5 g·kg^-1). Mouse twisting numbers induced by acetic acid and mouse pain threshold after heat stimulation on hot-plate tests were respectively performed to investigate the analgesic effect of S. rostratus. Result: The LD₅₀level of water extract of S. rostratus. was 68 g·kg^-1, the auricle edema induced by dimethylbenzene in mice,catton ball-induced granuloma in mice and carrageenan-induced hind paw edema in rats were inhibited significantly by water extract of S. rostratus. (P<0.05 or P<0.01), water extract of S. rostratus could remarkably reduce mouse twisting numbers (P<0.05 or P<0.01), but could not improve pain threshold. Conclusion: The water extract of S. rostratus had anti-inflammatory effect and analgesic effect,the researches could provide a scientific basis for developing and utilizing of S. rostratus.