蛋白质组学在肝损伤本质研究中的应用现状及策略

Status and Proposals of Applying Proteomics to Research Essence of Liver Injury

摘要:

查阅Pubmed、CNKI等国内外相关研究资料30篇并对其进行汇总和分析。①蛋白质组学技术已应用于药源性肝损伤(DILI)、四氯化碳(CCl₄)肝损伤、酒精性肝损伤、脂多糖(LPS)肝损伤、免疫性肝损伤、硫代乙酰胺肝损伤、放射性肝损伤、溴苯肝损伤、肼肝损伤、非酒精性脂肪性肝病(NAFLD)等多种类型肝损伤本质研究中,其中尤以在DILI中的应用最为多见;②测试用蛋白样品主要取自动物肝和血清,偶亦用到尿液、胆汁酸、线粒体等;③双向凝胶电泳(2-DE)、MALDI-TOF-MS(基质辅助激光解吸电离飞行时间质谱)和多级MS是其主要分析工具;④肝损伤的蛋白质组机制研究广度和深度有待加强;⑤药物保肝作用的蛋白质组本质研究相对较少。应加强蛋白质组学在肝损伤本质研究中应用的深度,以促进肝损伤本质的全面揭示;应加强蛋白质组学在药物保肝本质研究中的应用力度,从而为保肝药物的科学应用提供更深入更全面的科学依据。

Abstract:

To discuss status and proposals of applying proteomics to research essence of liver injury. Looking through the related literatures from home and abroad, then analyzing and compiling them.①Proteomics technique has been applied to many types of liver injury induced by drug, carbon tetrachloride (CCl₄), alcohol, lipopolysaccharides (LPS), immune related, thioacetamide, radiation, bromobenzene, hydrazine, or non-alcoholic fatty liver disease (NAFLD). ②The protein samples for analysis are mainly taken from the animal liver, serum, urine, bile acids, mitochondria, etc. ③Main analyzing tools of proteomics are two-dimensional gel electrophoresis (2-DE), Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) and multi-stage MS. ④Research on proteome mechanism of liver injury needs to be strengthened. ⑤Hepatoprotective effect of drugs is not enough through proteomics. We should strengthen researches on essence of liver injury and essence-based hepatoprotection of drugs through proteomics.