川芎挥发油中内酯类成分的稳定性考察

Investigation of Stability of Lactones in Volatile Oil from Chuanxiong Rhizoma

摘要:

目的: 考察川芎挥发油中内酯类成分的稳定性。方法: 采用HPLC分别于不同时间测定避光冷藏(4℃),室温 避光(18~25℃),室温不避光条件下川芎挥发油中藁本内酯的含量,计算藁本内酯的降解速率常数,色谱条件为Hedera ODS-2 色谱柱(4.6 mm×250 mm,5 μm),流动相甲醇-水(65:35),检测波长273 nm,柱温30℃,进样量10 μL,流速1 mL·min^-1。分析相同质量浓度下川芎内酯A、丁基苯酞和丁烯基苯酞的峰面积变化。结果: 在避光冷藏、室温避光、室温不避光3种条件下挥发油中藁本内酯的降解速率常数分别为2.0×10^-4,6.4×10^-3,8.4×10^-3;随贮存时间的延长,丁基苯酞和丁烯基苯酞含量逐渐升高,藁本内酯和川芎内酯A含量逐渐降低,且丁烯基苯酞含量升高幅度与藁本内酯含量降低幅度接近。结论: 川芎挥发油宜避光<4℃贮存,主要成分在贮存过程中可相互转化。

Abstract:

Objective: To invesitgate stability of lactones in volatile oil from Chuanxiong Rhizoma. Method: The content of ligustilide at different times was determined by HPLC when volatile oil was put in refrigeration(4℃) avoiding light, room temperature(18-25℃) avoiding light and room temperature under light,respectively.degradation rate constant of ligustilide was calculated,chromatographic conditions were as follows:Hedera ODS-2 column(4.6 mm×250 mm,5 μm),mobile phase of methanol-water(65:35),detection wavelength 273 nm,column temperature 30℃,injection volume 10 μL,flow rate 1 mL·min^-1.And analyzed peak area change of butylphthalide,3-butylidenephthalide and senkyunolide A at the same concentration. Result: Under these three conditions,degradation rate constants of ligustilide in volatile oil were 2.0×10^-4,6.4×10^-3,8.4×10^-3,respectively;With extension of storage time,contents of butylphthalide and 3-butylidenephthalide increased while ligustilide and senkyunolide A decreased,and increasing amplitude of 3-butylidenephthalide was close to reduction amplitude of ligustilide. Conclusion: Volatile oil from Chuanxiong Rhizoma should be stored below 4℃ avoiding light,and its components could transform into each other during storage.