预处理方法对羊胆汁中胆酸含量的影响

Effect of Pretreatment Methods on Content of Cholic Acid of Sheep Bile

摘要:

目的: 考察羊胆汁预处理方法对胆酸含量的影响。方法: 采用直接干燥法、皂化法、真空冷冻干燥法、离心法制备胆酸,通过紫外分光光度法测定胆酸含量,考察不同预处理方法对胆酸含量的影响。结果: 不同预处理方法对羊胆汁中胆酸含量影响较大,皂化后粗品、皂化后精品、冷冻干燥品、离心干燥品1、离心干燥品2、胆囊直接干燥品的胆酸质量分数分别为63.41%,79.55%,39.67%,30.62%,41.71%,36.26%。结论: 综合干燥品总得率及胆酸含量考虑,直接离心后加95%乙醇除去蛋白成分的方法和真空冷冻干燥法较好。

Abstract:

Objective: To investigate effect of pretreatment methods on the content of cholic acid from sheep bile. Method: Cholic acid was prepared by direct drying method,saponification method,vacuum freeze drying method,centrifugation method,respectively.The content of cholic acid was determined by UV,amd effect of pretreatment methods on the content of cholic acid from sheep bile was investigated. Result: Effect of different processing methods on the content of cholic acid was great,contents of cholic acid from saponification crude products,saponification refined products,freeze-dried products,centrifugation drying products1,centrifugation drying products2 and gallbladder direct drying products were 63.41%,79.55%,39.67%,30.62%,41.71%,36.26%,respectively. Conclusion: Comprehensive evaluation with yield of dry products and the content of cholic acid,direct centrifugation combining with adding 95% ethanol removal of protein composition and vacuum freeze drying method were better than other methods.