不同产地白头翁中白头翁皂苷D的含量测定

Determination of Pulsatilla Saponin D in Pulsatillae Radix from Different Areas

摘要:

目的: 建立白头翁中白头翁皂苷D的含量测定方法。方法: 采用HPLC测定白头翁皂苷D的含量,色谱条件为依利特Hypersil ODS₂ C₁₈色谱柱(4.6 mm×250 mm,5 μm),流动相乙腈-水-甲酸(38:62:0.1),流速1.0 mL·min^-1,检测波长203 nm,柱温25℃,考察不同产地白头翁中白头翁皂苷D的含量差异。结果: 白头翁皂苷D线性范围0.311 2~3.112 μg(r=0.999 9),精密度RSD 1.36%,重复性RSD 1.65%,供试品溶液在24 h内稳定,平均加样回收率98.07%(RSD 0.64%)。吉林产白头翁中白头翁皂苷D含量最高(0.256%),比广东产白头翁高了约20倍。结论: 该测定方法简便易行、结果准确、重复性好。不同产地的白头翁中白头翁皂苷D的含量差异较大,选用时应慎重。

Abstract:

Objective: To establish a determination method for pulsatilla saponin D in Pulsatillae Radix. Method: The content of pulsatilla saponin D was determined by RP-HPLC,chromatographic conditions were as follows:Elite Hypersil ODS₂ C₁₈ column(4.6 mm×250 mm,5 μm),mobile phase of acetonitrile-water-formic acid(38:62:0.1),flow rate 1.0 mL·min^-1,detection wavelength 203 nm,column temperature 25℃.Content difference of pulsatilla saponin D in Pulsatillae Radix from different areas was investigated. Result: Pulsatilla saponin D had good linear relationship in 0.311 2-3.112 μg(r=0.999 9),RSD of instrument precision was 1.36%,RSD of method repeatability was 1.65%,samples solution was stable within 24 h,the average recovery was 98.07%(RSD 0.64%).The content of pulsatilla saponin D in Pulsatillae Radix from Jilin was the highest(0.256%),which was 20 folds higher than Pulsatillae Radix from Guangdong. Conclusion: This method was simple,accurate and repeatable.The content of pulsatilla saponin D in Pulsatillae Radix from different areas had much difference,so materials choice should be careful.