HPLC同时测定茵栀黄颗粒中4个环烯醚萜苷类成分

Simultaneous Determination of Four Iridoid Glycosides in Yinzhihuang Granules by HPLC

摘要:

目的: 建立HPLC同时测定茵栀黄颗粒中栀子苷、山栀苷、去乙酰车叶草苷酸甲酯和京尼平-1-β-D-龙胆二糖苷4个环烯醚萜苷类成分含量的方法。方法: Kromasil C₁₈色谱柱(4.6 mm×250 mm,5 μm),流动相乙腈(A)-0.05%三氟乙酸溶液(B)梯度洗脱(0~15 min,5%~6%A;15~23 min,6%~10%A;23~35 min,10%~22%A),流速1.0 mL·min^-1,检测波长238 nm,柱温30℃。结果: 栀子苷、山栀苷、去乙酰车叶草苷酸甲酯和京尼平-1-β-D-龙胆二糖苷进样量分别在0.334~3.344,0.041 5~0.415,0.046 6~0.466,0.093 1~0.931 μg与峰面积呈良好线性关系,平均回收率分别为97.3%,99.6%,97.6%,99.2%,RSD分别为1.6%,2.7%,2.3%,2.2%。结论: 该分析方法简便、准确,可用于完善茵栀黄颗粒的定量分析方法,以提高该制剂的质量标准。

Abstract:

Objective: To develop an HPLC method for simultaneous determination of four iridoid glycosides (geniposide,shanzhiside,deacetyl asperulosidic acid methyl ester and genipin-1-β-D-gentiobioside) in Yinzhihuang granules. Method: Chromatographic conditions were as follows:Kromasil C₁₈ column (4.6 mm×250 mm,5 μm),mobile phase of acetonitrile(A)-0.05% trifluoroacetic acid solution(B) with gradient elution program(0-15 min,5%-6%A;15-23 min,6%-10%A;23-35 min,10%-22%A),flow rate 1.0 mL·min^-1,detection wavelength 238 nm,column temperature at 30℃. Result: The linear ranges of geniposide,shanzhiside,deacetyl asperulosidic acid methyl ester and genipin-1-β-D-gentiobioside were 0.334 4-3.344 0,0.041 5-0.415 2,0.046 6-0.466 4,0.093 1-0.931 2 μg,respectively;Average recoveries of these four ingredients were 97.3%(RSD 1.6%),99.6%(RSD 2.7%),97.6%(RSD 2.3%) and 99.2%(RSD 2.2%),respectively. Conclusion: This method was convenient and accurate,it could be used to improve quality standard of Yinzhihuang granules.