5种紫珠属药材中总酚、总黄酮与其抗氧化活性的相关性研究

Correlation of the Contents of Total Phenols and Total Flavonoids and Antioxidant Activities of Five Callicarpa Species

摘要:

目的: 研究5种紫珠属药材中总酚、总黄酮与其抗氧化活性的相关性。方法: 采用紫外分光光度法测定5种紫珠属药材总酚和总黄酮的含量;采用清除O₂^-·,DPPH·,ABTS⁺·,·OH评价法、Cu²⁺螯合法、Fe³⁺还原法及对大豆卵磷脂脂质过氧化的抑制效果评价它们之间抗氧化活性的差异。结果: 5种药材的总酚和总黄酮含量相差较大,其中,裸花紫珠含量最大;各种方法所得抗氧化活性强弱顺序有区别;除了抗脂质过氧化外,其他方法所得结果均与总酚和总黄酮含量呈正相关。结论: 5种紫珠属药材富含总酚和总黄酮成分,具有较强抗氧化活性,可以作为天然抗氧化剂进行开发。

Abstract:

Objective: To study the Correlation of the contents of total phenols and total flavonoids and antioxidant activities of five Callicarpa species. Method: The contents of total phenols,total flavonoids of five Callicarpa species were detected with Uv-Visible spectrophotometer. Subsequently, the antioxidant activities of five Callicarpa species were determined by seven antioxidant methods, including·O₂^- scavenging, DPPH·scavenging, ABTS⁺·scavenging,·OH scavenging, Cu²⁺-chelating, and Fe³⁺ reducing assays, anti-lipidperoxidation. Result: The contents of total phenols,total flavonoids of five Callicarpa species were significantly different with each other and Callicarpa nudiflora Hook. et Arn possessed the highest contents. There were various kinds of relationship about antioxidant activities of five Callicarpa species from seven methods. The results of correlation analysis indicated that there was a positive correlation between the contents of total phenols and total flavonoids and antioxidant activities apart from anti-lipidperoxidation activity. Conclusion: Five Callicarpa species were rich in phenols and flavonoids,had excellent antioxidant activities,so they would be developed as new natural antioxidants.