近红外光谱法快速检测玉竹酒基提取液中多糖的含量

Rapid Determination of the Polysaccharide Content in Polygonatum Wine Matrix Extract by Near Infrared Reflectance Spectroscopy

摘要:

目的: 建立测定玉竹酒基提取液中多糖含量的近红外光谱(NIR)快速分析方法。方法: 采用苯酚-硫酸法测定73批玉竹酒基提取液中多糖含量,采用傅里叶变换近红外透射光谱技术采集玉竹酒基多糖提取液的近红外光谱,结合偏最小二乘法(PLS)建立多糖的定量校正模型。结果: 玉竹酒基提取液多糖校正模型校正集的相关系数(R)、内部交叉验证均方差(RMSECV)分别为0.970 3,0.771,经外部验证,预测值与实测值的相关系数为0.969 2,预测均方差(RMSEP)为0.624。结论: 该方法快速方便、准确可靠,可用于玉竹酒基提取液多糖含量的快速测定。

Abstract:

Objective: To establish a near infrared spectroscopy method for the determination of polysaccharides in Polygonatum wine matrix extract. Method: The polysaccharide was measured by phenol-sulfuric acid method and the near-infrared spectroscopy of the Polygonatum wine matrix extract was collected by Fourier transform near infrared spectroscopy technology. Partial least square (PLS) was utilized to build the polysaccharide quantitative calibration model. Result: The results show that correlation coefficient (R), internal cross-validation mean square error (RMSECV) were 0.970 3 and 0.771.External validation samples collected on-line were used to evaluate the quantitative models. The root mean square error of prediction (RMSEP) and the correlation coefficient of the true value and prediction were 0.624 and 0.969 2. Conclusion: The presented method is rapid, accurate and reliable, which can be used in practice to fast determine polysaccharide content in Polygonatum wine matrix extract.