UPLC同时测定野菊花中蒙花苷和绿原酸含量

Simultaneous Determination of Chlorogenic Acid and Buddleoside in Chrysanthemi Indici Flos by UPLC

摘要:

目的: 建立UPLC同时测定不同产地野菊花中蒙花苷和绿原酸含量的分析方法。方法: 采用超高效液相色谱系统, C₁₈ 色谱柱(2.1 mm×50 mm,1.8 μm),流动相乙腈-0.1%磷酸水,以0.2 mL·min^-1的流速进行梯度洗脱,检测波长326 nm,柱温25℃。结果: 蒙花苷在3.0~120.0 mg·L^-1线性关系良好(r=1.000 0),平均回收率99.43%,RSD 1.54%(n=6);绿原酸在0.48~19.2 mg·L^-1线性关系良好(r=1.000 0),平均回收率98.67%,RSD 2.13%(n=6)。结论: UPLC法分析速度快,重复性好,结果准确且高效、简便,可用于野菊花中蒙花苷和绿原酸含量的测定。

Abstract:

Objective: To establish a UPLC method for simultaneous determination of buddleoside and chlorogenic acid in Chrysanthemi Indici Flos from different areas. Method: UPLC was used. The chromatographic separation was performed on a C₁₈ (2.1 mm×50 mm, 1.8 μm) column with gradient elute. The flow rate was 0.2 mL·min^-1. The detection wavelength was set at 326 nm and column temperature was maintained at 25℃. Result: Good linear relationship between the peak area and content of buddleoside and chlorogenic acid was achieved when the amount was within 3.0-120.0, 0.48-19.20 mg·L^-1;the recoveries were 99.43% and 98.67%;RSDs were 1.54% and 2.13% respectively(n=6). Conclusion: The UPLC analysis is speed, reproducible, accurate, efficient and convenient, which can detect the content of buddleoside and chlorogenic acid in Chrysanthemi Indici Flos.