小槐花提取物对α-葡萄糖苷酶抑制活性研究

α-Glucosidase Inhibitory Activity of Extracts from Desmodium caudatum

摘要:

目的: 评价小槐花Desmodium caudatum (Thunb.)DC.提取物对α-葡萄糖苷酶的抑制活性。方法: 通过建立体外α-葡萄糖苷酶抑制模型,对小槐花提取物进行活性筛选。结果: 小槐花提取物均有较好的α-葡萄糖酶抑制作用,其中以乙酸乙酯部位(DCEA) 和石油醚部位(DCPE) 活性较好(IC₅₀=9.79,39.2 mg·L^-1),其次为正丁醇部位 (DCBU) (IC₅₀=625.1 mg·L^-1),抑制活性均远远大于阳性对照阿卡波糖 (IC₅₀=1 103.01 mg·L^-1)。DCBU对α-葡萄糖苷酶抑制活性呈显浓度依赖性;DCPE和DCEA对α-葡萄糖苷酶抑制活性对浓度的变化较灵敏,随浓度的增加而急剧上升。结论: 小槐花石油醚部位和乙酸乙酯部位对α-葡萄糖苷酶活性抑制非常显著,具有开发治疗糖尿病降血糖药物的价值。

Abstract:

Objective: To evaluate α-glucosidase inhibitory activity of extracts from Desmodium caudatum. Method: By establishing α-glucosidase inhibitory model in vitro, the activity of extracts from D. caudatum was screened. Result: The extracts from D. caudatum showed good inhibitory activity. The ethyl acetate extract and petroleum ether extract had higher inhibitor activity (IC₅₀=9.79 and 39.2 mg·L^-1), which showed far greater activity than that of acarbose (IC₅₀=1 103.01 mg·L^-1), and followed by petroleum ether and n-butanol extract (IC₅₀=625.1 mg·L^-1). The α-glucosidase inhibitory activity of DCBU had concentration-dependent manner. α-Glucosidase inhibitory activity of DCPE and DCEA is sensitive to the change of concentration, which increased dramatically as the concentration increased. Conclusion: DCPE and DCEA showed very significant inhibitory activity to α-glucosidase, and could be exploited the diabetes drugs as α-glucosidases inhibitors in future.