秦巴山区中药材藿香枝叶中多糖研究

Study on the Polysaccharide of the Branch and Leaf of Folk Medicine Agastache rugosa Obtained from Qinba Mountain

摘要:

目的: 对中药材藿香枝叶中的多糖进行提取,并测试其抗氧化活性,为藿香药材资源的综合利用提供科学依据。方法: 采用超声法辅助提取藿香枝叶中的多糖,设计L₉(3⁴)正交试验考察料液比、pH和超声提取时间对藿香枝叶中多糖提取的影响,确定其多糖的最佳提取工艺,并对藿香多糖进行清除·OH试验。结果: 藿香多糖的最佳提取工艺为料液比1:10、超声提取30 min、pH为6,可使藿香多糖的提取率高达7.50%,藿香多糖对Fenton反应产生的·OH有明显的清除作用。结论: 藿香多糖具有抗氧化活性,可从藿香枝叶中提取多糖,其多糖有开发价值。

Abstract:

Objective: Agastache rugosa polysaccharide was extracted from the branches and leaves of A. rugosa, the research can provide and supply scientific basis for the search of its polysaccharide antioxidant activity to empolder A. rugosa medicinal materials. Method: The polysaccharide was obtained from A. rugosa branches and leaves by ultrasonic extraction method, and tested L₉(3⁴) orthogonal experiment on the base of material-liquid ratio, ultrasonic time and pH value, to optimize A. rugosa polysaccharide extract technology;A. rugosa polysaccharide to remove hydroxyl radical was also studied. Result: The best A. rugosa polysaccharide extracting technology assisted by ultrasonic was material-liquid ratio 1:10, ultrasonic time 30 min, and pH value 6, and the extraction rate of polysaccharide was reached up to 7.50%. A. rugosa polysaccharide had significant scavenging effect for hydroxyl radical produced by Fenton reaction theory. Conclusion: A. rugosa polysaccharide had antioxidant activity. The polysaccharide may be extracted from the branches and leaves of A. rugosa, and had development value.