香橼、佛手中黄酮类成分含量测定及HPLC特征图谱研究

Determinnaiton of Flavonoids and Study on HPLC Characteristic Spectrum of Citrus Fructus and Citrus Sarcodactylis Fructus

摘要:

目的: 建立香橼、佛手药材中黄酮类成分的含量测定方法及HPLC特征图谱。方法: 采用高效液相色谱法对特征图谱进行研究,并对橙皮苷和柚皮苷进行含量测定,色谱柱为Waters SunFire^TM C₁₈(4.6 mm×150 mm,5 μm),流动相乙腈-0.1%磷酸梯度洗脱,检测波长284 nm,流速1.0 mL·min^-1,柱温30℃。结果: 所建立的特征图谱可同时用于含量测定,香橼、佛手黄酮类成分分离度良好,柚皮苷线性范围为0.190 4~1.142 4 μg(r=0.999 5),平均加样回收率为102.98%(n=6);橙皮苷线性范围为0.173 6~1.215 2 μg(r=0.999 5),枸橼中橙皮苷平均加样回收率为101.19%(n=6),佛手中橙皮苷平均回收率为99.11%(n=6)。结论: 所建立的特征图谱及含量测定方法快速、准确、可靠,为香橼、佛手药材的质量控制方法提供科学依据,可用于香橼、佛手质量的科学评价。

Abstract:

Objective: To establish an HPLC method for determination and characteristic spectrum of flavonoids in Citrus Fructus and Citri Sarcodactylis Fructus. Method: Chromatographic analysis was carried on the coloumn of Waters SunFire^TM C₁₈(4.6 mm×150 mm, 5 μm), the mobile phase was acetonitrile-0.1% phosphoric acid at the flow rate of 1.0 mL·min^-1;the detection wavelength was set at 284 nm and the coloumn temperature was 30℃. Result: The flavonoids compounds were base-isolated in HPLC characteristic spectrum. The linear range of hesperidin was 0.173 6-1.215 2 μg(r=0.999 5), the average recovery was 101.19%(n=6) in Citrus medica, and the average recovery was 99.11%(n=6) in Citri Sarcodactylis Fructus. The linear range of naringin was 0.190 4-1.142 4 μg(r=0.999 5), the average recovery was 102.98%(n=6). Conclusion: The method is rapid, accurate and reliable, and can be applied to the quality control of Citrus Fructus and Citri Sarcodactylis Fructus.