引种栝楼过氧化物同工酶分析

POD Isozyme Analysis of Introduced Trichosanthes kirilowii from Different Prodution Regions

摘要:

目的: 对国内不同产区栝楼进行种源间亲缘关系的分析。方法: 采用烯酰胺凝胶电泳(PAGE)技术对不同种源栝楼过氧化物同工酶(POD)进行研究。结果: 不同产区栝楼过氧化物同工酶在酶带数、迁移率和酶量等方面存在差异。栝楼POD酶谱共有10条谱带,分为5个类群,Ⅰ,Ⅴ和其他4个类群亲缘关系都较远,Ⅱ,Ⅲ,Ⅳ这3个类群的亲缘关系较近。结论: 过氧化物同工酶谱可以作为不同种源栝楼遗传多样性鉴定的参考依据。

Abstract:

Objective: To study the germplasm resources of Trichosanthes kirilowii. Method: The experiment was analyzed by using the technique of peroxidase(POD) isozyme. Result: The results show that obvious differences exist in the number of bands,Rf and amount of POD isozyme.The enzyme spectrum own 10 species, divided into five compartments. Ⅰ and Ⅴ are distantly related to other 3 species。Ⅱ,Ⅲ and Ⅳ are closed to each other. Conclusion: POD isozyme patterns can be a useful marker for genetic diversity of T. kirilowii from different regions.