藏药吉尔巴对糖尿病性视网膜病变的影响及机制研究

Study on the Effect of Berberis dictyophlla Cortex on Diabetic Retinopathy and the Mechanism

摘要:

目的: 研究藏药吉尔巴对糖尿病视网膜病变模型(DR)大鼠的降血糖作用、抗氧化应激作用、血管保护作用及其相关机制。方法: SD大鼠单次ip给予链脲佐菌素(STZ)40 mg·kg^-1制备2型糖尿病模型,高脂饲料喂养5个月,随机分组,给予相应药物,连续30 d,实验期间监测大鼠体质量、血糖的变化,实验结束后检测大鼠超氧化物歧化酶(SOD)活力,一氧化氮(NO)、丙二醛(MDA)、细胞黏附分子(ICAM-1)、血管紧张素Ⅱ(ANGⅡ)的水平,并通过大鼠视网膜HE染色分析其病理变化。结果: 吉尔巴浸膏可显著降低DR模型大鼠空腹血糖,可减轻视网膜病理改变,降低大鼠视网膜内皮细胞/周细胞值(E/P),显著增强SOD活力,降低血清细胞黏附分子(ICAM-1)和血管紧张素Ⅱ(ANGⅡ)含量。结论: 吉尔巴浸膏能够减轻糖尿病视网膜病变程度,减缓发展进程,同时具有整体调节功能,对糖尿病大鼠视网膜具有保护作用。

Abstract:

Objective: To evaluate the hypoglycemiceffect of extract from Tibetan medicine Berberis dictyophlla Cortex on postprandial blood glucose (PBG), protection against oxidative stress, protection function on blood vessels in diabetic rats induced by streptozotocin(STZ)and to explore the mechanism. Method: The type 2 diabetic rat model was established by ip injection of STZ (40 mg·kg^-1) on SD rats and the rats were fed with high sugar and high fat for 5 months.Then, the rats were divided into 8 groups and administered. During the experiment, the body weight, fasting blood glucose (FBG) were measured. After 30 days of administration, the superoxide dismutase (SOD), malondialdehyde (MDA), nitric oxide(NO), cell adhesion molecule(ICAM-1), angiotensin(ANGⅡ), and other blood biochemical indexes were measured. Result: The B. dictyophlla cortex could reduce significantly the level of fasting blood glucose,ICAM-1 and ANGⅡexpression. The retinas were taken and observed by histopathological analysis, the tissue injury attenuated compared with the model group. The contents of SOD in serum of group were higher while MDA was lower than the model group. Conclusion: DR of B. dictyophlla cortex group was obviously better compared with the model group. The extract of B. dictyophlla cortex can improve effectively retinopathy of the rats, relieve the degree and development of DR and regulate function in the whole. All in all, Berberis Cortex showed the effect of prevention and therapy of DR.