螺旋藻多糖对胃溃疡大鼠胃黏膜的修复作用及对EGF和EGFR表达的影响

Effect of Polysaccharide from Spriulnia platensis on Mucosal Repair by Regulating the Expression of EGF and EGFR in Experimental Rats with Gastric Ulcer

摘要:

目的: 观察螺旋藻多糖(PSP) 对胃溃疡大鼠胃黏膜表皮生长因子(EGF)及表皮生长因子受体(EGFR)表达及溃疡愈合质量的影响,探讨其治疗胃溃疡的作用机制。方法: 采用乙酸烧灼法制备实验性胃溃疡大鼠模型,给予高、低两个剂量的PSP干预治疗15 d后取材,HE染色观察胃黏膜厚度、黏膜肌层缺损度变化;免疫组化观察胃溃疡大鼠胃黏膜EGF及EGFR表达情况。结果: PSP对胃溃疡黏膜有一定的修复作用,可使溃疡细胞破损减少,胃黏膜厚度、黏膜肌层缺损度与模型组比较均有显著性差异,免疫组化可见PSP使胃溃疡大鼠胃黏膜EGF及EGFR表达上调。结论: PSP治疗胃溃疡的作用机制可能与增加EGF及EGFR蛋白表达上调促进溃疡愈合有关。

Abstract:

Objective: To investigate the effect of polysaccharide from Spriulnia platensis(PSP)on expression of epidermal growth factor(EGF) and EGF receptor(EGFR) in mucosa of experimental rats with gastric ulcer, and to study the possible mechanism for therapy of stomach ulcer. Method: The gastric ulcer in rats was induced by acetic acid burning and treated with low and high dose of PSP separately for 15 days, the gastric mucosal thickness and muscularis mucosa defect were observed by HE staining, and the expression of EGF and EGFR were detected by immunohistochemical staining. Result: The ulcer in treatment group healed well compared with model group and control group, high dose of PSP could promote the healing of gastric ulcer, reduce muscularis mucosa defect significantly (P<0.01), increased the expression of EGF and EGFR observably. Conclusion: There is evident therapeutic effect of PSP on the experimental gastric ulcer in rats, and its mechanism might be related to the increasing EGF and EGFR protein expression.