香附油滴丸抗炎镇痛作用及其物质基础研究

Study on Anti-inflammatory and Analgesic Activities of Xiangfuyou Diwan and its Material Basis

摘要:

目的: 探讨香附油滴丸抗炎镇痛作用及其物质基础,为香附油滴丸运用于防治妇科痛经疾病提供依据。方法: 采用角叉菜胶致小鼠足跖肿胀、二甲苯致小鼠耳廓肿胀及醋酸致小鼠腹腔毛细血管通透性升高模型,考察香附油滴丸的抗炎作用;运用醋酸致小鼠扭体、热刺激致小鼠疼痛模型,考察香附油滴丸的镇痛作用,并与阿司匹林和四制香附丸比较。采用高效液相色谱法检测香附油滴丸灌胃给药后在大鼠血清中的移行成分,考察其抗炎镇痛作用的可能物质基础。结果: 香附油滴丸连续5 d ig给药,100, 200 mg·kg^-1组均可显著减轻小鼠足跖及耳廓肿胀,降低小鼠腹腔毛细血管通透性,提高小鼠扭体法、热板法致疼痛的痛阈,香附油滴丸在血清中的移行成分主要为α-香附酮。结论: 香附油滴丸具有明显的抗炎镇痛作用,其物质基础可能主要是α-香附酮。

Abstract:

Objective: To discuss the anti-inflammatory and analgesic activities of Xiangfuyou Diwan (XYD) and its material basis, and to provide basis for its application in gynecological dysmenorrhea disease. Method: Carrageenan-induced mouse footpad edema, xylene-induced mouse ear edema and acetic acid-induced increased mouse vascular permeability were used to study the anti-inflammatory activity of XYD. And its analgesic activity was evaluated in mouse models of pain induced by acetic acid and thermal stimulus. The above activities were compared with those of Aspirin and Sizhi Xiangfu Wan. Meanwhile, high performance liquid chromatography was used to explore its material basis by assaying the transitional ingredients in rat blood after administered orally with XYD. Result: XYD significantly inhibited carrageenan-induced footpad edema and xylene-induced ear edema at the doses of 100, 200 mg·kg^-1 after oral administration for 5 days. Meanwhile, XYD markedly reduced acetic acid-induced dye leakage from vascular. On the other hand, XYD remarkably suppressed acetic acid-induced writhing response at low, moderate and high doses, and significantly prolonged pain threshold in hot plate assay at moderate and high doses. Overall, its potency was similar to that of Aspirin and Sizhi Xiangfu Wan. Above all, as the main transitional ingredient in XYD, α-cyperone was detected in medicinal serum. Conclusion: XYD has significant anti-inflammatory and analgesic activities, and its main material basis may be α-cyperone.