基因芯片技术检测消瘤保肺丸对肿瘤转移相关基因表达的影响

Effect of Xiaoliu Baofei Pill on Tumor Metastasis-associated Gene Expression based on Gene-chip Technology

摘要:

目的: 基因芯片技术检测消瘤保肺丸对肿瘤转移相关基因表达的影响。方法: 采用消瘤保肺丸中药灌胃、采集兔含药血清。消瘤保肺丸含药血清与PG细胞培养48 h后,观察用药后细胞肿瘤转移相关基因表达的变化。结果: PG细胞空白组与用药组比较:经药物干预后,用药组有30多个基因表达上调大于1.5倍,其中包括DCC,GPNMB,MCAM,ETV4,FN1等,无表达下调基因。结论: 消瘤保肺丸对肺癌转移的调控是多层次、多靶点的;同时也反映出该药对于转移潜能不同的细胞类型其作用靶点可能也不尽相同。

Abstract:

Objective: The gene-chip technology was used to detect effect of Xiaoliu Baofei pill on tumor metastasis-associated gene expression. Method: The serum containing Xiaoliu Baofei pills in vitro was used to observe PG cells tumor metastasis-associated gene expression. Result: Compared with the treatment group PG cells in the blank group upregulated the expressionof more than 30 genes, including cell cycle genes,cell adhesion genes,cell growth and proliferation genes,apoptosis genes,transshipment,and control genes,matrix metalloproteinase protein and other metastasis-related genes. No down-regulated genes were found. Conclusion: Regulation of Xiaoliu Baofei pill on lung cancer metastasis is a multi-level and multi-targeted.