四逆散对溃疡性结肠炎大鼠ICAM-1和VCAM-1的影响

Influence of Sinisan on ICAM-1 and VCAM-1 in Experimental Ulcerative Colitis Rats

摘要:

目的: 探讨四逆散对实验性溃疡性结肠炎(UC)大鼠细胞间黏附分子(ICAM)-1和血管内皮细胞黏附分子(VCAM)-1的影响,以及君臣配伍在组方中的作用。方法: SD大鼠50只,分为5组,即正常组、模型组、四逆散组、柴芍枳组和柴芍组;采用免疫法造模,用家兔新鲜结肠黏膜制备抗原乳化液,分别于造模第1,10, 17, 24天注射于大鼠的双侧足跖、腹股沟及背部皮内,造模第2天分组给药,正常组与模型组按照10 mL·kg^-1蒸馏水ig;四逆散组按照1.75 g·kg^-1四逆散溶液ig;柴芍枳组按照1.31 g·kg^-1 ig;柴芍组按照0.88 g·kg^-1 ig;第29天给药后,取材,处死大鼠;采用双抗体夹心ELISA法检测大鼠血清及结肠黏膜组织ICAM-1和VCAM-1水平。结果: 模型组大鼠血清ICAM-1(64.60±4.81)μg·L^-1明显高于正常组(42.35±3.02)μg·L^-1,P<0.05;VCAM-1(0.29±0.01)μg·L^-1显著高于正常组(0.20±0.02)μg·L^-1,P<0.05;模型组大鼠结肠黏膜组织VCAM-1(1.82±0.19)μg·mg^-1显著高于正常组(1.29±0.10)μg·mg^-1,P<0.05;经灌服四逆散后,实验大鼠血清ICAM-1(53.87±2.60)μg·L^-1、VCAM-1(0.22±0.01)μg·L^-1,结肠黏膜组织VCAM-1(1.41±0.08)μg·mg^-1含量均较模型组显著降低,P<0.05。结论: 四逆散能够抑制UC大鼠炎性细胞浸润,减轻炎症反应,其作用机制可能与抑制实验大鼠ICAM-1和VCAM-1的表达有关,方中发挥主要作用的药物可能为柴胡、枳实和芍药。

Abstract:

Objective: To study Sinisan on intercellular adhesion molecule-1(ICAM-1) and vascular cell adhesionmolecule-1(VCAM-1) in experimental (UC) rats. Method: The animals were divided into 5 groups, including the normal group, model group, Sini powder group, Chaishaozhi group and Chaishao group. The antigen emulsion made by fresh rabbit colonic mucosa were injected into the rats' bilateral paws, groins and backs in 1st, 10th, 17th, 24th day to induce model. The normal group and model group were given with 10 mL·kg^-1 distilled water;and Sinisan group was treated with 1.75 g·kg^-1 Sinisan solution;and Chaishaozhi group was treated with Chaishaozhi 1.31 g·kg^-1 Chaishaozhi solution;and Chaishao group was treated with with 0.88 g·kg^-1 Chaishao solution. On the 29th day, the rats were sacrificed and drawn. ICAM-1 and VCAM-1 levels were detected with ELISA. Result: ICAM-1 levels in blood of model group(64.60±4.81)μg·L^-1 were higher than normal group(42.35±3.02)μg·L^-1,P<0.05, and VCAM-1 levels(0.29±0.01)μg·L^-1 higher than normal(0.20±0.02)μg·L^-1,P<0.05.VCAM-1 levels in colon mucosal tissue of model group(1.82±0.19)μg·mg^-1 higher than normal group(1.29±0.10)μg·mg^-1,P<0.05.Sini san could significantly reduce ICAM-1(53.87±2.60)μg·L^-1, VCAM-1(0.22±0.01)μg·L^-1 levels in blood,and VCAM-1(1.41±0.08)μg·mg^-1in colon mucosal tissue, P<0.05. Conclusion: The mechanism of intervening experimental UC by Sinisan is related to the balance of cytokine network.