通窍化栓汤对脑缺血再灌注模型大鼠脑组织超微结构的影响

Effects of Tongqiao Huashuan Decoction on Brain Tissue Ultrastructure of Bolt in Rates with Cerebral Ischemia

摘要:

目的: 观察不同剂量的通窍化栓汤对大鼠局灶性脑缺血再灌注损伤模型脑组织超微结构的影响,并探讨其可能的作用机制,为临床通窍化栓汤治疗急性脑梗死提供实验依据。方法: 将SD大鼠随机分为5组:通窍化栓汤高剂量组、中剂量组、低剂量组、模型组、假手术组。采用可逆性大脑中动脉线栓法制备成局灶性脑缺血再灌注模型(MCAO大鼠模型),给予相应的剂量按17.50,8.75,4.35 g·kg^-1·d^-1、生理盐水治疗15 d后,观察脑组织中超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量变化及脑组织超微结构变化。结果: 与假手术组比较,模型组MDA含量显著增加,SOD活性显著降低(P<0.05);与模型组相比,通窍化栓汤各剂量组均能降低脑组织中MDA含量,升高SOD活性,有极显著差异(P<0.01)。通窍化栓汤高剂量组与中、低剂量组相比有显著性差异(P<0.05),并能够减轻脑组织损伤后细胞凋亡。结论: 高剂量通窍化栓汤能明显减轻神经细胞变性坏死,减少细胞凋亡,从而改善神经功能缺损,其机制可能与清除自由基有关。

Abstract:

Objective: To observe the effect of Miao medicine Tongqiao Huashuan decoction on brain tissue ultrastructure of bolt in rats with focal cerebral ischemia/reperfusion injurys. Method: SD rats were randomly divided into 5 groups (Tongqiao Huashuan decoction high dosage group, middle dose group,low dose group, model group,sham operation group).The middle cerebral artery occlusion (MCAO)was used to establish focal cerebral ischemia-reperfusion model. The corresponding Tongqiao Huashuan decoction (17.50,8.75,4.35 g·kg^-1·d^-1),saline gavage were given for 15 days. The brain tissue superoxide dismutase (SOD) activity, malondialdehyde (MDA) content and ultrastructure of brain tissue were observed. Result: Compared with the sham operation group, MDA in model group was significantly increased, SOD activity was significantly decreased (P<0.05);compared with the model group, Tongqiao Huashuan decoction reduced the MDA content in brain tissues, increased the activity of SOD (P<0.01). Tongqiao Huashuan decoction could reduce the damage of brain tissue after cell apoptosis. Conclusion: High doses of Tongqiao Huashuan decoction could obviously alleviate the degeneration and necrosis of nerve cells and reduce apoptosis,its mechanism may be related to eliminate free radicals.