旋覆代赭汤对RE模型大鼠食管黏膜Na+-K+-ATP酶及 Ca2+-Mg2+-ATP酶的影响

Effects of Xuanfu Daizhe Decoction on Na+-K+-ATPase and Ca2+-Mg2+- ATPase Activity of Esophageal Mucosa in Reflux Esophagitis Rats

摘要:

目的: 观察旋覆代赭汤对反流性食管炎(RE)模型大鼠食管黏膜组织Na⁺-K⁺-ATP酶及Ca²⁺-Mg²⁺-ATP酶活性的影响,探讨RE食管黏膜细胞能量代谢障碍与黏膜损伤的关系以及旋覆代赭汤治疗RE的作用机制。方法: 将96只雄性Wistar大鼠随机分成6组,即假手术组、模型组、旋覆代赭汤组高、中、低剂量组(24,12,6 g·kg^-1)及西药对照组(奥美拉唑10 mg·kg^-1+2 mg·kg^-1莫沙必利),每组16只。采用"食管-十二指肠端侧吻合术"制备胃及十二指肠液混合RE模型。从术后第3天开始,假手术组、模型组给予生理盐水灌胃,药物治疗组分别给予旋覆代赭汤高、中、低剂量、西药(奥美拉唑10 mg·kg^-1+莫沙必利2 mg·kg^-1)灌胃,连续7天,记录大鼠体重变化及死亡情况,于第8天处死大鼠留取标本,观察食管下段黏膜大体及病理组织学变化;化学法检测食管黏膜组织Na⁺-K⁺-ATP和Ca²⁺-Mg²⁺-ATP酶活性。结果: 与假手术组比,模型组大鼠体重明显减轻(P<0.05),食管黏膜肉眼及镜下病理改变明显,评分增高(P<0.05);食管黏膜组织Na⁺-K⁺-ATP酶及Ca²⁺-Mg²⁺-ATP酶活性显著降低(P<0.05);与模型组及西药组比,旋覆代赭汤高、中剂量组大鼠死亡率明显降低(P<0.05):与模型组比,旋覆代赭汤高、中剂量组、西药组肉眼及病理评分显著降低(P<0.05);食管黏膜组织Na⁺-K⁺-ATP酶及Ca²⁺-Mg²⁺-ATP酶活性明显提高(P<0.05)。结论: 旋覆代赭汤能明显提高RE大鼠食管黏膜细胞膜Na⁺-K⁺-ATP 酶及Ca²⁺-Mg²⁺-ATP酶活性, 从而保持食管黏膜细胞完整性,减轻黏膜损伤。

Abstract:

Objective: To observe Na⁺-K⁺-ATPase and Ca²⁺-Mg²⁺-ATPase activity of esophageal mucosa in reflux esophagitis(RE) rats, and to investigate the fundamental pathogenesis of RE and the therapeutic mechanism in treating RE with Xuanfu Daizhe decoction. Method: Ninty six male Wistar rats were divided into 6 groups:sham operation group, model group, Xuanfu Daizhe decoction high, middle and low dose group (24,12,6 g·kg^-1)and western medicine (OME 10 mg·kg^-1 plus Mosapride 2 mg·kg^-1) group, 16 rats each group. Esophagus-duodenum anastomosis was used to establish rat RE model. From 3 days after operation, NS was given to the sham operation group rats and model group rats by gavage feeding;Xuanfu Daizhe decoction and western medicine (OME 10 mg·kg^-1 plus mosapride 2 mg·kg^-1) was given to the medicine treated groups, and execute all rats in the 8^th day, then observe mucous gross change of lower esophagus with eyes and optical microscope. At last, detect the expression of enzymatic activity of Na⁺-K⁺-ATP and Ca²⁺-Mg²⁺-ATP by chemical methods. Result: Compared with sham operation group, the mortality of the model group was higher;the pathological changes and score were increased;the Na⁺-K⁺-ATPase and Ca²⁺-Mg²⁺-ATPase of esophageal mucosa were decreased. The mortality of the high and middle dose group of Xuanfu Daizhe decocton treated group was decreased significantly compared with the model group (P<0.05). The rats weight of Xuanfu Daizhe decoction treated group were increased significantly compared with the model group (P<0.05). The pathological changes and score were all reduced in Xuanfu Daizhe decoction high and middle dose group(P<0.01);both Na⁺-K⁺-ATPase and Ca²⁺-Mg²⁺-ATPase of esophageal mucosa were increased in Xuanfu Daizhe decoction high and middle dose group (P<0.01). Conclusion: Xuanfu Daizhe decoction could increase the activity of Na⁺-K⁺-ATPase and Ca²⁺-Mg²⁺-ATPase, and protects the cellular integrity of esophageal mucosa, reducing the esophageal mucosal injury of RE.