基于因子分析的定经汤调节卵巢早衰生物学机制研究

Study on Biological Mechanisms of Premature Ovarian Failure in Rats by Curing with Dingjing Decoction Based on Factor Analysis

摘要:

目的: 探讨定经汤调节卵巢早衰的生物学机制。方法: SD大鼠90只,随机分为3组:正常组、模型组、定经汤干预组;分别检测其与卵巢早衰相关的生物分子:β-内啡肽 (β-EP) 、白介素-1 (IL-1)、一氧化氮合酶(NOS)、一氧化氮(NO)、雌激素受体 (ER)、孕激素受体(PR)、促性腺激素释放激素 (GnRH)、促肾上腺皮质激素释放激素 (CRH)、促卵泡激素(FSH)、黄体生成素(LH)、雌二醇(E₂)、孕酮(P)、促肾上腺皮质激素(ACTH)和皮质醇(CORT);利用因子分析方法探究定经汤干预下各生物分子之间的相互调控关系,并探寻定经汤调节卵巢早衰过程中的关键生物分子。结果: 定经汤干预状态的生殖内分泌网络主调控因子主要包括垂体-卵巢轴上的4个生物分子,垂体-肾上腺轴靶腺层生物分子CORT以及部分下丘脑生物分子;IL-1是定经汤干预状态生殖内分泌网络主调控因子与其他公共因子共有的下丘脑部位的生物分子。结论: 定经汤一方面可以直接调节垂体-肾上腺轴生物分子,另一方面又可以通过下丘脑间接调节垂体-肾上腺轴生物分子;各调控因子之间起枢纽作用的生物分子为IL-1。

Abstract:

Objective: Explore the biological mechanisms of the premature ovarian failure in rats by curing with Dingjing decoction. Method: Ninty SD rats were randomly divided into three groups:normal groups, model groups and Dingjing decoction groups;biomolecules which associated with premature ovarian failure:β-endorphin(β-EP), interleukin-1(IL-1),nitric oxide synthase(NOS),nitric oxide synthase(NO), estrogen receptor(ER), progesterone receptor(PR), gonadotropin releasing hormone(GnRH), corticotropin releasing hormone(CRH),follicle-stimulating hormone(FSH), luteinizing hormone(LH), estradiol(E₂₎, progesterone(P), adrenocor ticotropic hormore(ACTH) and cortisol(CORT)were detected. Regulation relations of biological molecules were explored in curing by Dingjing decoction based on factor analysis. And to explore the key biomolecules in premature ovarian failure by curing with Dingjing decoction. Result: The master regulatory factor mainly contains 4 pituitary-ovarian axis biomolecules, pituitary-adrenal axis biomolecule CORT and parts of hypothalamus biomolecules in curing state by Dingjing decoction. And IL-1 is common biomolecules in the hypothalamus part of the curing state's first and second common factor. Conclusion: On the one hand, Dingjing decoction can adjust the pituitary-adrenal axis molecules directly, on the other hand, it can indirectly adjust the pituitary-adrenal axis molecules by hypothalamus. Among the factors, the molecules IL-1 play a pivotal role.