蒙药"连翘-4味汤散"对幽门结扎性肝损伤的保护作用

Protective Effect of Mongolian Medicine ’Lianqiao-4 Ingredient Soup Powder’ on Liver Injury Induced by Pylorus Ligation

摘要:

目的: 研究蒙药"连翘-4味汤散"对大鼠幽门结扎引起急性肝损伤的影响。方法: 采用幽门结扎法制备大鼠急性肝损伤模型。造模16 h后分别检测正常组、模型组、蒙药连翘-4味汤散低、中、高剂量组(1.3,2.6,3.9 g·kg^-1)ig给药,连续10 d后血清丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)、单胺氧化酶(MAO)、一氧化氮(NO)、一氧化氮合酶(NOS)、胆碱酯酶(CHE)、尿素氮(BUN)、总胆固醇(CHO)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)的水平以及肝指数和胃匀浆胃蛋白酶活性。结果: 与正常组比较,模型组血清AST,ALT,BUN,NOS均明显增高(均P<0.01),CHE,NO明显降低(P <0.05),MAO,CHO,TG,LDL-C含量肝脏指数和胃蛋白酶活性均明显增高(P<0.05);与模型组比较,连翘-4味汤散中、高剂量组血清AST,ALT,NOS,MAO,CHO,TG,LDL-C水平及肝脏指数和胃蛋白酶活性均有明显降低(P<0.05,P<0.01),CHE,NO明显增高(分别为P<0.05),低剂量组对各种指标有好转趋势,但无显著差异。模型组呈肝细胞萎缩、重度脂肪变性、水肿;连翘-4味汤散中剂量组与正常组基本接近,高剂量组有少量脂肪变和轻度水肿,低剂量组与模型组无明显差异。结论: 蒙成药连翘-4味汤散对幽门结扎引起的急性肝损伤具有明显的保护作用,且中剂量效果最好。

Abstract:

Objective: To evaluate the effect of Mongolian medicine ‘Lianqiao-4 Ingredient Soup Powder' on the acute liver injury of rats caused by pylorus ligation. Method: Model of acute liver injury was induced by using pylorus ligation. After making model of 16 h indexes of alanine aminotransferase(ALT), aspartate aminotransferase(AST), nitric oxide(NO), nitric oxide composite enzyme(NOS), cholinesterase(CHE), urea nitrogen(URE), total cholesterol(CHO), triglyceride(TG), low density lipoprotein(LDL-C)in serum, liver index and pepsin in the stomach homogenate in the normal group, model group, Mongolian medicine ‘Lianqiao-4 Ingredient Soup Powder' low, middle, high dose groups(1.3, 2.6, 3.9 g·kg^-1)were determined respectively. Result: Compared with normal group, serum AST, ALT, BUN, NOS in the model group were increased significantly (all P<0.01);CHE, NO decreased significantly (P<0.05);MAO, CHO, TG, LDL-C level, liver index and activity of pepsin increased significantly (P<0.05). Compared with model group, AST, ALT, NOS, MAO, CHO, TG, LDL-C level, liver index and activity of pepsin in the high dose group of treatment were decreased significantly (P<0.05, P<0.01 respectively), CHE and NO increased significantly (P<0.05). Low dose showed some influences for all indexes, but without significant difference compared with model group. Conclusion: Significant protective action of Mongolian medicine 'Lianqiao-4 Ingredient Soup Powder' on the acute liver injury was found. And taking various elements into the comprehensive consideration, the middle dose group was the best.