乳络通胶囊对小鼠乳腺增生模型的影响

Effect of Ruluotong Capsules on Mammary Gland Hyperplasia in Mice

摘要:

目的: 建立小鼠乳腺增生模型,观察乳络通胶囊(乳络通)对小鼠乳腺增生模型的血清雌二醇(E₂)、孕酮(P)、脏器(胸腺、脾脏和子宫)指数及乳腺组织形态学变化的影响,探讨乳络通对小鼠乳腺增生模型的作用机制。方法: 将雌性小鼠随机分为对照组、模型组、乳络通高、中、低剂量(0.6,0.3,0.15 g·kg^-1)、乳康片(0.53 g·kg^-1)组。采用苯甲酸雌二醇和黄体酮建立小鼠乳腺增生模型,治疗组分别用乳络通高、中、低剂量、乳康片连续灌药30 d。放射免疫法测定血清E₂,P的含量、脏器(胸腺、脾脏和子宫)指数,常规HE染色,光镜下观察乳腺组织形态的变化及其采用立体定量分析方法观察乳腺小叶体密度、间质体密度和腺泡体表面。结果: 乳络通高、中、低剂量可显著降低小鼠乳腺增生模型血清中E₂含量(P<0.01),明显升高P含量(P<0.05);乳络通高剂量组胸腺指数显著减小(P<0.01),子宫指数显著增大(P<0.01);病理形态学显示乳络通高、中、低剂量组的乳腺小叶体密度、腺泡比表面都显著减小(P<0.01),小叶间质体密度显著增大(P<0.01),乳络通组乳腺病变减轻,有抑制乳腺小叶增生的作用,使小叶体积缩小,腺泡缩小,导管变窄。结论: 乳络通可通过调节乳腺增生小鼠的雌激素水平,改善增生乳腺的病理形态变化,有效地对抗雌激素引起的小鼠乳腺增生。

Abstract:

Objective: To establish a mice model of mammary gland hyperplasia, to observe the effects of Ruluotong on estradiol (E₂), progesterone (P), visceral index,and pathologic changes of breast tissue in mammary gland of the model mice, and to study the mechanism of the actions. Method: Female mice were divided into blank control group,model control group,Ruluotong capsules high, midlle and low doses group (0.6,0.3,0.15 g·kg^-1),Rukang tables group(0.53 g·kg^-1). Model of mammary glands hyperplasia in mice was established by taking estradiol benzoate and progesterone. The treatment groups were given with different doses of Ruluotong capsules and Rukang pills for thirty days. The contents of E₂, P of serum and visceral index were determined,pathologic changes of breast tissue were observed. Result: Ruluotong high,medlle and low doses could significantly decrease E₂ level(P<0.01) of model serum and increase the P content of serum(P<0.05). Thymus index in the high dose group was markedly(P<0.01)and womb index was significantly increased(P<0.01). Pathomorphological investigation showed three doses of Ruluotong could significantly reduce lobular breast density and alveolar surface area(P<0.01),and increase lobular interstitial density(P<0.01). Pathological changes were mitigated by the capsules,with inhibition of mammary lobular hyperplasia,showing shrink in lobular size,narrowed acinar and duct. Conclusion: Ruluotong capsules can improve the pathological changes of the mammary gland hyperplasia by regulating proliferation of breast estrogen levels in mice to inhibit mammary gland hyperplasia of mice induced by estrogen effectively.