Box-Behnken设计-效应面法优选防风炮制工艺

Optimization of Processing Technology for Saposhnikoviae Radix by Box-Behnken Design-Response Surface Methodology

摘要:

目的：优选防风的炮制工艺。方法：以升麻素苷和5-O-甲基维斯阿米醇苷含量、浸膏得率、水分的综合评分为指标，采用Box-Behnken设计-效应面法考察切片厚度、干燥时间、干燥温度对防风饮片质量的影响，优选防风饮片的炮制工艺参数。结果：最佳炮制工艺为切片厚度3~4 mm，干燥温度60 ℃，干燥时间2 h。升麻素苷和5-O-甲基维斯阿米醇苷的质量分数分别为0.917%，0.054%，水分5.110%，浸膏得率20.45%。结论：采用Box-Behnken设计-效应面法优选的炮制工艺合理可行，为防风饮片的质量控制提供参考。

Abstract:

Objective: To optimize processing technology of Saposhnikoviae Radix. Method: Taking composite score of contents of prim-O-glucosylcimifugin and 5-O-methylvisammioside,extract yield,water contents as index,effects of slice thickness,drying time and drying temperature on pieces quality were investigated by Box-Behnken design-response surface methodology,so as to optimize processing technology parameters of Saposhnikoviae Radix. Result: Optimal processing parameters were as follows:slice thickness 3-4 mm,drying temperature at 60 ℃,drying time 2 hours.Under these conditions,contents of prim-O-glucosylcimifugin and 5-O-methylvisammioside,extract yield,water contents were 0.917%,0.054%,20.45% and 5.110%,respectively. Conclusion: Optimized processing technology for Saposhnikoviae Radix was feasible and reasonable,it could provide a reference for quality control of Saposhnikoviae Radix pieces.